大豆ERF转录因子基因GmERF8的克隆与表达分析  

Cloning and Expression Analysis of ERF Transcription Factor Gm ERF8 in Soybean(Glycine max L.)

作　　者：翟莹[1] 张军[2] 赵艳[1] 任巍巍[1] 张闯[1] 孙婉姝[1] 高士童 ZHAI Ying ZHANG Jun ZHAO Yan REN Wei-wei ZHANG Chuang SUN Wan-shu l GAO Shi-tong(College of Life Science and Agro-forestry, Qiqihar Univesity, Qiqihar 161006 Heilongfiang Institute of Veterinary Science, Qiqihar 161005)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006 [2]黑龙江省兽医科学研究所,齐齐哈尔161005

出　　处：《植物遗传资源学报》2016年第6期1036-1040,共5页Journal of Plant Genetic Resources

基　　金：国家自然科学基金项目(31301335);黑龙江省教育厅科学技术研究项目(12541889);黑龙江省自然科学基金项目(C201458)

摘　　要：ERF转录因子广泛存在于植物中并且参与植物应对生物及非生物胁迫的响应。本研究利用RT-PCR技术从大豆中克隆获得1个新的ERF转录因子基因Gm ERF8,开放阅读框全长627 bp,编码1个由208个氨基酸残基组成的分子量为23.43 k D的蛋白。蛋白结构预测发现,该蛋白含有1个典型的AP2/ERF结合域,2个预测的核定位信号和1个保守的EAR抑制元件。进化分析表明Gm ERF8蛋白与烟草Nt ERF3蛋白的同源性最高。实时荧光定量PCR表明,Gm ERF8在大豆的根和叶中表达量较高。ABA、高盐和低温处理均使Gm ERF8表达量下降;乙烯(ET)和干旱处理则使Gm ERF8的表达量先下降后升高。转录调节能力分析结果显示,Gm ERF8可以抑制报告基因的表达。上述实验结果表明,Gm ERF8可能作为转录抑制子参与大豆对环境胁迫的应答。ERF transcription factors are widespread in plants,which are widely involved in plant response to biotic and abiotic stress. In this research,a gene Gm ERF8 encoding a new ERF protein was isolated using RT-PCR from soybean. Gm ERF8 consisted of an ORF（open reading frame） with length of 627 bp,and encoded a 23. 43 k D protein with 208 amino acids. Bioinformatics analysis indicated that Gm ERF8 contained a typical AP2/ERF binding domain,two putative nuclear localization signal sequences and a conserved repression-associated EAR motif. The amino acid sequences of Gm ERF8 and Nt ERF3 shared high homology through phylogenetic analysis. Real-time fluorescence quantitative PCR results revealed that Gm ERF8 expressed highly in roots and leaves. The expression of Gm ERF8 decreased under ABA,salt and cold treatments. Whereas it decreased firstly and thereafter increased under ethylene and drought treatments. Transcription regulation experiments demonstrated that Gm ERF8 down-regulated the transcriptional level of the reporter gene. As the result,Gm ERF8 may response to environmental stress as a transcriptional repressor in soybean.

关 键 词：大豆 ERF转录因子 GM ERF8 表达分析 抑制子 

分 类 号：Q943.2[生物学—植物学]