糖分子探针与炎症因子、Claudin-8在结肠炎大鼠肠黏膜通透性改变中的相关性  

Correlation of Sugar Molecular Probes with Inflammatory Factors and Claudin-8 in Intestinal Permeability in Colitis Rats

作　　者：施慧[1,2] 王震凯[1] 王少东[1] 路又可[1] 汪芳裕[1] 

机构地区：[1]南京军区南京总医院消化内科,210002 [2]南方医科大学南京临床学院

出　　处：《胃肠病学》2016年第10期606-609,共4页Chinese Journal of Gastroenterology

基　　金：南京军区南京总医院青年课题基金(批准号:2008021);中国博士后科学基金面上资助(201150M1575);中国博士后科学基金特别资助(2012T50895)

摘　　要：背景:肠黏膜通透性改变是溃疡性结肠炎发展的重要环节。糖分子探针是检测通透性的一种安全无创的方法,其与炎症因子、claudin-8在黏膜通透性改变中的相关性尚不明确。目的:探讨糖分子探针与TNF-α、CRP和claudin-8在结肠炎大鼠肠黏膜通透性改变中的相关性。方法:24只大鼠随机分为模型组和正常对照组,以DSS溶液制备结肠炎模型。ELISA法检测TNF-α、CRP表达,免疫组化法检测claudin-8表达,高效液相色谱仪检测糖分子探针含量,分析炎症因子、claudin-8与糖分子探针的相关性。结果:与正常对照组相比,模型组结肠组织TNF-α、CRP表达显著升高(P<0.01);claudin-8表达显著降低(P<0.01);乳果糖和三氯蔗糖排出量以及乳果糖/甘露醇比值明显升高(P<0.01),甘露醇排出量明显降低(P<0.01)。乳果糖、三氯蔗糖排出量与TNF-α、CRP表达呈正相关(P<0.01),与claudin-8表达呈负相关(P<0.01);而乳果糖/甘露醇比值、甘露醇排出量与TNF-α、CRP表达呈负相关(P<0.01),与claudin-8表达呈正相关(P<0.01)。结论:糖分子探针与TNF-α、CRP以及claudin-8表达在提示大鼠肠黏膜通透性改变上的结果一致。糖分子探针可作为一种反映肠黏膜通透性改变的潜在检测方法。Background： Intestinal permeability plays an important role in the development of ulcerative colitis. Sugar molecular probes is a safe and non-invasive method to measure intestinal permeability,and its correlation with inflammatory factors and claudin-8 is not clear. Aims： To explore the correlation of sugar molecular probes with TNF-α,CRP and claudin-8 in intestinal permeability in colitis rats. Methods： Twenty-four rats were assigned randomly to model group and normal control group. Colitis model was induced by DSS solution. Expressions of TNF-α,CRP were determined by ELISA, and expression of claudin-8 was determined by immunohistochemistry. Sugar molecular probes were determined by high performance liquid chromatography. Correlations of inflammatory factors and claudin-8 with sugar molecular probes were analyzed. Results： Compared with normal control group,expressions of colonic tissue TNF-α and CRP were significantly increased while expression of claudin-8 was significantly decreased in model group（ p〈0. 01）; secretion of lactulose and sucrolose,ratio of lactulose / mannitol（ L / M） were significantly increased（ p〈0. 01） while mannitol secretion was significantly decreased（ p〈0. 01）. Secretion of lactulose and sucrolose were positively correlated with expressions of TNF-α and CRP（ p〈0. 01）,but negatively with expression of claudin-8（ p〈0. 01）. L /M ratio and mannitol secretion were negatively correlated with expressions of TNF-α and CRP（ p〈0. 01）,but positively with expression of claudin-8（ p〈0. 01）. Conclusions： Sugar molecular probes and expressions of TNF-α,CRP,claudin-8 have similar results in predicting intestinal permeability in rats. Sugar molecular probes can be used as a potential method to measure intestinal permeability.

关 键 词：通透性 分子探针 肿瘤坏死因子α C反应蛋白质 claudin-8 结肠炎 溃疡性 

分 类 号：R574.62[医药卫生—消化系统]