Reprimo基因3'非翻译区839位点多态性与肺癌发生的关联  

Study of Reprimo 839 G>C polymorphism and relationship with lung cancer

作　　者：陈贤华[1] 胡宏波[1] 冯金[1] 孙秀娟[2] 刘大鹰[3] 

机构地区：[1]广西省柳州市柳铁中心医院检验科,广西柳州545007 [2]广西省柳州市柳铁中心医院病理科,广西柳州545007 [3]广西省柳州市柳铁中心医院呼吸内科,广西柳州545007

出　　处：《中国现代医学杂志》2016年第20期16-20,共5页China Journal of Modern Medicine

基　　金：柳州市科学技术局技术研究与开发计划课题(No:2011J0302031);广西卫生厅计划课题(No:Z2010138)

摘　　要：目的探讨Reprimo基因3'非翻译区839位点单核苷酸多态性改变与肺癌发生的关系。方法基因分型用TAKARA的Mighty Amp DNA聚合酶法,分别检测36例肺癌患者(癌症组)和23例非癌患者(对照组)的Reprimo基因3'非翻译区839位点G>C分布,运用χ2检验、Logistic回归分析和Armitage’s趋势检验等分析基因多态性、患者性别、吸烟与否和年龄与肺癌发生的相关性。结果两组标本经Hardy-Weinberg平衡检测,对照组在Reprimo基因3'非翻译区839位点G>C基因型观测(理论)频数为0,22,1(5.26,11.48,6.26),差异有统计学意义(P=0.000),癌症组的基因型观测(理论)频数为6,29,1(11.67,17.65,6.67),差异有统计学意义(P=0.000);经Logistic回归分析,基因多态性、年龄是危险因素,基因多态性、年龄和吸烟与否在肺癌发生中不存在交互作用(P>0.05);经Armitage’s趋势检验差异无统计学意义(χ2=3.56,P=0.059)。结论 Reprimo基因3'非翻译区839位点G>C单核苷酸位点多态性与肺癌发生具有一定的相关性。Objective To investigate the relationship between the Reprimo gene 3＇-untranslated region 839 polymorphism and lung cancer. Methods Genotyping was performed with MightyAmp DNA Polymerase, a number of 36 lung cancer cases and 23 control cases were genotyped. The results of investigation were analyzed with χ^2 test, Logistic regression analysis and Armitage＇s trend test. Results According to Hardy-Weinberg equilibrium detection, Reprimo 839 G〉C genotype observation （theory） frequency was 0, 22, 1 （5.26, 11.48, 6.26） in the control group （with statistically significant difference, P = 0.000） and 6, 29, 1 （11.67, 17.65, 6.67） in the cancer group （with statistically significant difference, P = 0.000）. Analyzed by Logistic regression analysis, gene polymorphism and age were risk factors, and there were no possible interactions among gene polymorphism, age, smoking status and lung cancer （P 〉 0.05）. Armitage＇s trend test revealed no statistically significant correlation between 839 G〉C polymorphism and susceptibility to lung cancer （χ^2 = 3.56, P = 0.059）. Conclusions This study indicates that there is a correlation between the Reprimo gene 3＇-untranslated region 839 G〉C polymorphism and lung cancer.

关 键 词：肺癌 Reprimo 多态性 哈迪一温伯格平衡 

分 类 号：R734.2[医药卫生—肿瘤]