趋化因子配体12、趋化因子受体7在肠型胃癌中的表达与淋巴结和肝脏转移的关系  

Expression of chemokine ligand 12/chemokine receptor type 7 in intestinal type gastric cancer and its relationship with lymph node and liver metastasis

作　　者：辛琪[1] 张勤[1] 张娜 温丽坤[1] 刘贵秋[1] 张传山[1] 战忠利[3] 

机构地区：[1]天津市第三中心医院病理科天津市人工细胞重点实验室,300170 [2]天津市滨海新区大港医院病理科 [3]天津市肿瘤医院病理科

出　　处：《中华消化杂志》2016年第11期740-745,共6页Chinese Journal of Digestion

基　　金：天津市滨海新区卫生局医药卫生科技项目（2011BHKY021）;天津市滨海新区大港区域社会发展科技项目（20120211）;天津市滨海新区科技发展战略研究计划项目（2012DK15W007）

摘　　要：目的探讨趋化因子配体（CXCL）12/趋化因子受体7（CXCR7）生物学轴在肠型胃癌中的表达与临床病理特征和淋巴结、肝脏转移的关系。方法选取肠型胃癌及其配对癌旁组织60份，肠型胃癌转移淋巴结30份，肠型胃癌肝转移组织20份作为研究对象。采用免疫组织化学SP法检测胃癌及其癌旁组织、转移淋巴结和肝转移组织中CXCL12和CXCR7的表达。分析CXCL12、CXCR7在肠型胃癌中的表达与临床病理特征的关系，以及其与淋巴结、肝脏转移的关系。进一步分析CXCL12＋CXCR7＋、CXCL12＋CXCR7－或CXCL12－CXCR7＋、CXCL12－CXCR7－胃癌组织与肿瘤大小、浸润深度、临床分期的关系，以及与淋巴结、肝脏转移的关系。统计学采用McNemar检验、卡方检验、Spearman等级相关分析。结果CXCR7在肠型胃癌中表达，并与CXCL12呈正相关关系（Kappa＝0.321，P＝0.010）。CXCR7在肠型胃癌中的表达，与淋巴结转移、肝转移、肿瘤大小、浸润深度、临床分期有关（χ2＝5.879、7.547、5.701、7.699、4.434，P均〈0.05）。淋巴结转移、肝转移组织中CXCL12＋CXCR7＋细胞表达更多（r＝0.586，P〈0.01；r＝0.275，P＝0.033）；肿瘤最大径≥5 cm、浸润深度为T3＋T4、临床分期为Ⅲ＋Ⅳ期的肠型胃癌CXCL12＋CXCR7＋的表达更强。结论CXCL12/CXCR7可能作为生物学轴促进肠型胃癌生长浸润，促进其淋巴结和肝脏转移。ObjectiveTo investigate the expression of chemokine ligand 12（CXCL12）/chemokine receptor type 7（CXCR7） in intestinal type gastric cancer and its relationship with clinical pathological characteristics and lymph node, liver metastasis.MethodsSixty cases of intestinal type gastric cancer and its carcinoma adjacent tissues, 30 cases of intestinal type gastric cancer with lymph node metastasis and 20 cases with liver metastasis were selected as study subjects. The expression of CXCL12 and CXCR7 in gastric cancer tissues, its adjacent tissues and metastatic lymph nodes and liver tissues was detected by immunohistochemistry.The relationship between CXCL12, CXCR7 expression in intestinal type gastric cancer and clinical pathological characteristics, lymph node and liver metastasis were analyzed. The relationship between CXCL12＋ CXCR7＋ , CXCL12＋ CXCR7- or CXCL12-CXCR7＋ , CXCL12-CXCR7-gastric cancer tissue and tumor size, depth of invasion, clinical stage as well as lymph node, and liver metastasis were further analyzed. McNemar test, Chi square test and Spearman rank correlation analysis were performed for statistical analysis.ResultsCXCR7 expressed in intestinal type gastric carcinoma and was positively correlated with the expression of CXCL12 （Kappa=0.321, P=0.010）. The expression of CXCR7 in intestinal type gastric carcinoma was correlated with lymph node and liver metastasis, tumor size, depth of invasion and clinical stage （χ2=5.879, 7.547, 5.701, 7.699 and 4.434, all P〈0.05）. Lymph node and liver metastasis were more common in CXCL12＋ CXCR7＋ gastric cancer tissues than those of CXCL12＋ CXCR7-, CXCL12-CXCR7＋ , and CXCL12-CXCR7- gastric cancer （r=0.586, P〈0.01; r=0.275, P=0.033）. The expression of CXCL12＋ CXCR7＋ were stronger in tumor maxium diameter no less than 5 cm, depth of invasion T3＋ T4 and clinical stage Ⅲ＋ Ⅳ intestinal type gastric cancer.ConclusionCXCL12/CXCR7 could be an biological axis in proliferation, invasion, as well as lymph node and liver metastas

关 键 词：肠型胃癌 趋化因子CXCL12 CXCR7蛋白 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]