破裂颅内动脉瘤患者动脉瘤壁AIF-1、MMP-9和COX-2表达上调  

Upregulation of aneurysm wall AIF-1, MMP-9 and COX-2 expression in patients with raptured intracranial aneurysm

作　　者：刘小飞[1] 段永红[1] 廖勇仕[1] 唐文俊[1] 宋洪涛[1] 梁日初[1] 

机构地区：[1]南华大学附属第二医院神经外科,衡阳421001

出　　处：《国际脑血管病杂志》2016年第9期833-838,共6页International Journal of Cerebrovascular Diseases

基　　金：湖南省卫计委医药卫生科研计划课题B类项目（B2014-053）

摘　　要：目的探讨同种异体移植物炎症因子-1（allograft inflammatory factor-1，AIF-1）、基质金属蛋白酶-9（matrixmeta Uoproteinase-9，MMP-9）和环加氧酶-2（cycloxy genase-2，COX-2）在颅内破裂动脉瘤组织中的表达及意义。方法收集12例颅内破裂囊状动脉瘤瘤体和颞浅动脉标本，采用免疫组织化学染色法检测动脉瘤和颞浅动脉AIF．1、MMP-9和COX-2表达。结果动脉瘤瘤壁可见淋巴细胞、巨噬细胞等炎性细胞浸润。AIF-1、MMP-9和COX-2表达主要见于破裂动脉瘤瘤壁组织的内膜层和中膜层，并且主要分布于巨噬细胞和淋巴细胞的细胞质内，而在颞浅动脉不表达或极少量表达。半定量分析显示，动脉瘤瘤壁AIF—l破裂IAAIF．1[0．0067（0．0042～0．0146）对0．000o（o．0000～0．0010）；Z=-4．236，P〈0．001]、MMP-9[0．0022（0．0007～0．0043）对0．000o（o．0000～0．0000）；Z=-4．442，P〈0．001]和COX-2[0．0028（0．0022～0．0043）对0．0000（0．0000～0．0000）；z=-4．442，P〈0．001]表达水平均显著高于颞浅动脉组。男性患者破裂动脉瘤瘤壁AIF-1表达水平显著高于女性患者（0．016±0．013对0．009±0．006；t=1．440，P=0．043）。结论颅内破裂囊状动脉瘤瘤壁AIF-1、MMP-9和COX-2表达显著上调，提示存在炎性反应，并可能参与了颅内动脉瘤发生、发展和破裂过程。Objective To investigate the expression and significance of allograft inflammatory factor-1 （AIF-1）, matrix metalloproteinase-9 （MMP-9）, and cycloxy genase-2 （COX-2） in ruptured intracranial aneurysm tissue. Methods The aneurysm and superficial temporal artery specimens were collected from 12 patients with intracranial ruptured cystic aneurysms. Immunohistochemical staining was used to detect the expression levels of AIF-1, MMP-9 and COX-2 in the aneurysms and superficial temporal arteries. Results Inflammatory cell inf＇fltratinn such as lymphocytes, macrophages were observed in the aneurysmal walls. The AIF-1, MMP-9 and COX-2 expression levels were mainly observed in the intima layer and intima-media of the ruptured aneurysm wall tissue, and they were mainly distributed in the cytoplasms of macrophages and lymphocytes, and they did not express or expressed very few in superficial temporal arteries. Semi-quantitative analysis showed that expression levels of AIF-1 （0. 006 7[0. 004 2-0. 014 6]vs. 0.0000[0.0000-0.0010]; Z= 4.236, P〈 0.001）, MMP-9 （0.0022[0.0007-0.0043 ]vs. 0.0000[0.0000-0.0000]; Z= -4. 442, P〈 0.001）, and COX-2 （0.002 8[0.002 2-0.0043]vs. 0. 000 0[0. 000 0-0. 000 0] ; Z = -4. 442, P 〈0. 001） in the aneurysmal wall were significantly higher than those in the superficial temporal artery. The expression level of AIF-1 in male patients was significantly higher than that in female patients （0. 016 ± 0. 013 vs. 0. 009 ±0. 006; t = 1. 440, P =0. 043）. Conclusions The expression levels of AIF-1, MMP-9 and COX-2 of the ruptured intracranial saccular aneurysm walls were upregulated significantly, suggesting inflammatory response, and they might be involved in the occurrence, development and rupture process of intracranial aneurysms.

关 键 词：颅内动脉瘤 动脉瘤 破裂 钙结合蛋白质类 基质金属蛋白酶9 环加氧酶2 炎症 颞动脉 AIFl蛋白 人 

分 类 号：R743[医药卫生—神经病学与精神病学]