microRNA-21表达水平对新疆地区食管癌患者早期诊断及预后评估的临床价值  

Clinical Value of MicroRNA-21 Expression in Early Diagnosis and Prognosis of Esophageal Cancer in Xinjiang

作　　者：吕红博[1] 何占鳌 王洪江[1] 孙伟[1] 庞作良[1] 

机构地区：[1]新疆医科大学附属肿瘤医院胸外科,新疆乌鲁木齐830011

出　　处：《现代生物医学进展》2016年第31期6055-6059,共5页Progress in Modern Biomedicine

基　　金：新疆维吾尔自治区科技厅科技支疆项目(201491175)

摘　　要：目的:探讨microRNA-21表达水平对新疆地区食管癌早期诊断及预后评估的临床价值。方法:收集我院收治的100例食管癌患者,其中哈萨克族患者50例,汉族患者50例,采用RT-PCR以及RT-qPCR的方法对患者血清microRNA-21表达水平进行检测并比较。结果:食管癌患者癌组织中microRNA-21表达水平均高于癌旁组织,差异具有统计学意义(P<0.05);有淋巴结转移、ⅡB+Ⅲ期、低分化癌组织中的miRNA-21相对表达量高于无淋巴结转移、I+ⅡA期以及高分化癌组织患者,差异具有统计学意义(P<0.05);miRNA-21在不同民族、性别、年龄分组中表达无明显差异(P>0.05)。结论:microRNA-21表达水平对新疆地区食管癌患者癌组织中表达水平较高,在有淋巴转移、ⅡB+Ⅲ期以及低分化癌组织中表达水平较高,民族、性别以及年龄对microRNA-21水平的影响较小,因此microRNA-21检测对食管癌的早期诊断及预后判断具有指导意义。Objective： To investigate the value of microRNA-21 expression in the early diagnosis and prognosis of esophageal cancer in Xinjiang. Methods： 100 cases with esophageal cancer who were treated in our hospital were selected, including 50 Kazakhs and50 Han populations. Then the expression of microRNA-21 in the serum of patients was detected by RT-PCR and RT q PCR methods. Results：The expression of microRNA-21 in cancer tissues was higher than that of paracancerous tissues, and the difference was statistically significant（P 〈0.05）; The expression of miRNA-21 in patients with lymph node metastasis was higher tan that of the patients without lymph node metastasis（P〈0.05）; The expression of miRNA-21 in patients with ⅡB＋Ⅲ tumor stage was higher than that of the patients with I＋ⅡA tumor stage（P〈0.05）; The expression of miRNA-21 in patients high differentiated adenocarcinoma was lower than that of the patients with low differentiated adenocarcinoma（P〈0.05）; There was no statistically significant about the expression of miRNA-21 in patients with different nationalities, gender and ages（P〉0.05）. Conclusions： The expression of microRNA-21 of Xinjiang patients with esophageal cancer was higher in lymph node metastasis, ⅡB＋Ⅲ tumor stage and low differentiated adenocarcinoma. Thus, the detection of microRNA-21 is valuable for the diagnosis and prognosis of esophageal cancer.

关 键 词：MICRORNA-21 食管癌 新疆地区 

分 类 号：R735.1[医药卫生—肿瘤]