急性肺水肿大鼠肺组织中IL-6,SOD和MDA的变化  

Study on SOD,MDA and IL-6 in a rat model of acute pulmonary edema

作　　者：王娟[1] 贾合磊 吉红亮 任冬冬[1] 卢长青[1] 

机构地区：[1]河南中医药大学第二临床医学院急诊科,郑州450002 [2]郑州市郑荣医院普内科,郑州450002

出　　处：《中国比较医学杂志》2016年第11期38-42,共5页Chinese Journal of Comparative Medicine

摘　　要：目的探讨急性缺氧致大鼠急性肺水肿模型肺组织中丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)和白细胞介素-6(Interleukin 6,IL-6)的变化及其意义。方法成年Wistar大鼠50只,随机分为A组(正常组)、B组(急性肺水肿模型,缺氧24 h)、C组(急性肺水肿模型,缺氧48 h),D组(急性肺水肿模型,缺氧72 h)和E组(急性肺水肿激素治疗组,缺氧72 h)。对B,C,D,E组大鼠腹腔内注射6%氯化氨建立急性肺水肿模型,E组在注入6%氯化氨30 min后经尾静脉给药,给予地塞米松6.0 mg/kg。造模成功后的24 h,48h,72 h,分别处死4组大鼠,分离大鼠血浆,摘取大鼠肺脏,制备10%肺组织匀浆,用ELISA检测肺组织中MDA、SOD,IL-6和血浆中IL-6的含量和活性。结果 B,C,D三组可见大鼠肺组织水肿明显,湿重明显增加,与A组相比,差异有统计学意义(P<0.05)。A组大鼠肺组织无明显充血水肿,肺组织形态大致正常。B,C,D大鼠模型肺组织充血水肿,肺组织间质间隙、肺泡和细支气管内充满含有蛋白质的液体,肺泡内有透明膜形成。且D组大鼠肺组织充血水肿最为明显。而E组大鼠全肺组织水肿普遍较轻,HE染色显示E组大鼠肺组织肺泡融合,间隔较小,水肿液大多已吸收,肺泡内散在少量嗜伊红液体。B组与A组比较,肺组织中MDA,IL-6升高、SOD降低,但差异无统计学意义(P>0.05),随着肺水肿时间的延长,C,D组大鼠肺组织中MDA升高,SOD降低,血浆中的IL-6升高,和A组相比变化明显,差异有统计学意义(P<0.05)。和D组相比,E组大鼠肺组织中MDA降低,SOD升高,血浆中的IL-6降低,差异有统计学意义(P<0.05)。结论急性肺水肿的发生和氧化应激有关,机体抗氧化能力降低、自由基增加是肺水肿发生的重要机制,肺组织中IL-6,SOD和MDA含量对病情变化有指导意义。Objective To investigate Interleukin 6（ IL-6）,superoxide dismutase（ SOD）,malondialdehyde（ MDA） in lung tissues of a rat acute pulmonary edema model induced by acute hypoxia. Methods 50 adult Wistar rats were randomly divided into group A（ normal）,B（ acute pulmonary edema,hypoxia for 24 h）,C（ acute pulmonary edema,hypoxia for 48h）,D（ acute pulmonary edema,hypoxia for 72 h） and E（ dexamethasone treatment,hypoxia for 72h）. The model of acute pulmonary edema was established by intraperitoneal injection with 6% ammonium chloride. In group E,dexamethasone（ 6mg / kg） was injected in tail vein following intraperitoneal injection of 6% ammonium chloride. The rats were killed 24（ group B）,48（ C） and 72 hours（ DE） later. Plasma was isolated and lungs were removed. MDA,SOD in lung tissues and IL-6 in plasma were analyzed by ELISA. Results The wet weight of lung tissues were significantly increased in group B,C and D compared to group A（ P 0. 05）. The lung tissues of group A had no obvious congestion and edema,and the morphology of lung tissues was normal（ P 0. 05）. Pulmonary edema,interstitial spaces and alveolar filled fluid can be seen in group B,C,D and transparent membrane formed within alveoli. The lung tissue congestion and edema in group D were the most obvious（ P 0. 05）. The above changes obviously improved in group E. Compared with group A,MDA,IL-6 increased and SOD decreased in lung tissues of group C and D. Compared with group D,MDA was decreased and SOD was increased in lung tissues of group E and IL-6 was also decreased significantly in plasma（ P 0. 05）. Conclusion The occurrence of acute pulmonary edema related to oxidative stress and decreased antioxidant capacity. Increased free radical is an important mechanism of pulmonary edema. SOD,MDA and IL-6 in the lung tissue may be indexes for the prognosis.

关 键 词：急性肺水肿 大鼠模型 丙二醛 超氧化物歧化酶 白细胞介素-6 

分 类 号：R-332[医药卫生]