皮肤再生医疗技术对大鼠慢性难愈合溃疡创面ERK1/2、ATF2表达的影响  

Effects of skin regenerative medicine and therapy on ERK1/2,ATF2 expressions in rats with chronic hard-to-heal ulcer wounds

作　　者：唐乾利[1] 李利青[2] 何晓微[3] 曾鸿孟 王澍[1] 舒清锋 葛斌[1] 赵曦[1,3] 谢思圳 

机构地区：[1]右江民族医学院/桂西高发病防治重点实验室,广西百色533000 [2]湖南中医药大学,湖南长沙410208 [3]广西中医药大学,广西南宁530001

出　　处：《右江民族医学院学报》2016年第5期457-462,共6页Journal of Youjiang Medical University for Nationalities

基　　金：2014年广西自然科学基金面上项目(2014GXNSFAA118135);2015年国家自然科学基金项目(81560776)

摘　　要：目的动态观察大鼠难愈性创面组织ERK1/2、ATF2蛋白表达变化,从磷酸化ERK1/2对激活ATF2的交互作用(crosstalk)角度,阐明皮肤再生医疗技术(Moist exposed burn therapy/Moist exposed burn ointment,MEBT/MEBO)促进慢性难愈合创面修复的分子机制。方法 96只SD大鼠随机分为MEBO组、贝复济(rb-bFGF)组、模型组、空白对照组4组,各24只,创建体表皮肤溃疡模型,观察创面愈合情况,并于造模后第3d、7d、14d各取相同部位创面组织,采用Western Blotting技术检测磷酸化ERK1/2、ATF2蛋白表达水平。结果 MEBO组可见淡红色新生上皮由四周向创面中心生长,创面恢复良好。MEBO组和贝复济组创面愈合时间差异无统计学意义(P>0.05),均小于模型组(P<0.01);经14d治疗后,磷酸化ERK1/2及ATF2含量增高,同组内不同时间点ERK1/2、ATF2的表达差异有统计学意义(P<0.01),同时间点内不同组间的磷酸化ERK1/2、ATF2的表达差异有统计学意义(P<0.01),组间比较MEBO组和贝复济组磷酸化ERK1/2、ATF2的表达差异无统计学意义(P>0.05),但与模型组相比差异有统计学意义(P<0.01)。结论 MEBT/MEBO能加快大鼠慢性难愈合创面的愈合速度,提高修复质量;MEBT/MEBO可能通过激活ERK1/2信号通路,调控磷酸化ERK1/2、ATF2表达水平,使ERK1/2激活p38信号通路的下游底物ATF2产生交互作用,共同促进慢性难愈合创面的修复。Objective This article aims to dynamically observe extracellular signal-regulated kinase 1/2 （ERK1/2）,activated transcription factor 2 （ATF2）protein expression changes in rats’hard-to-heal wound tissues,and to elucidate the molecular mechanism of skin regenerative medicine and therapy （Moist exposed burn therapy/Moist exposed burn ointment,MEBT/MEBO）in enhancing hard-to-heal ulcer repair from the crosstalk of phosphorylated ERK1/2 activating ATF2. Methods Ninety-six SD rats were randomly divided into 4 groups：MEBO group,rb-bFGF group,model group,and blank control group,24 rats in each group. The superficial skin ulcer models were developed.The healing of wounds was observed.On days 3,7 and 14 after the models were developed,the same location tissues in every rats were collected,then the phosphoryla-ted ERK1/2,ATF2 protein expressions were measured by using Western Blotting. Results The newly pink epitheliums grew from periphery of wounds towards center.The recovery of wounds was fine.Comparison of wound healing time between MEBO group and rb-bFGF group showed there was no statistically significant difference （P〉0.05）,and both of MEBO group and rb-bFGF group had shorter wound healing time than that of model group （P〈0.01）.After 14-day treatment,the amount of phosphorylated ERK1/2 and ATF2 elevated;at different time points in the same group the expressions of ERK1/2,ATF2 were statistically differ-ent （P 〈0.01）;at the same time points in different groups the expressions of ERK1/2,ATF2 were statisti-cally different （P〈 0.01 ）;comparison of phosphorylated ERK1/2 and ATF2 expressions between MEBO group and rb-bFGF group showed there was no statistical difference （P 〉0.05 ）,but comparing to model group yielded statistical difference （P 〈0.01）. Conclusion MEBT/MEBO can accelerate the healing process of rats chronic hard-to-heal wounds and improve the healing quality.MEBT/MEBO may regulate phospho-rylated ERK1/2,ATF2 expressions by activating ERK1/2 signaling pathway whi

关 键 词：MEBT/MEBO 慢性难愈创面 交互作用 信号通路 

分 类 号：R-332[医药卫生]