高效液相色谱法同时测定人参制剂中20种人参皂苷方法的建立  

Simultaneous Determination of Twenty Ginsenosides in Ginseng Preparations by HPLC

作　　者：杨艳文[1] 孟凡双[1] 郜玉钢[1] 张连学[1] YANG Yanwen MENG Fanshuang GAO Yugang ZHANG Lianxue(College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China)

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《食品科学》2016年第22期131-135,共5页Food Science

基　　金：国家重点研发计划项目(2016YFC0500303);国家公益性行业(农业)科研专项(201303111);吉林省基础研究项目(20130102075JC);吉林省重点科技成果转化项目(20160307005YY;20140311050YY;20140307012YY)

摘　　要：为了更加有效评价人参制剂生产质量,建立了一种同时测定人参制剂中20种人参皂苷的高效液相色谱方法。结果表明,20种人参皂苷Rg1、Re、Rg2、Rg3、Rg5、Rf、F1、F2、Rc、Rd、Rb1、Rb2、Rb3、Rh2、compound K、20(R)-Rh1、Rk3、Rh4、原人参二醇及原人参三醇均得到良好分离,线性关系良好(R≥0.999 2)。该方法快捷简便、稳定可靠,能够精确全面检测分析人参皂苷含量,对于人参加工品及其制剂的质量控制更为全面准确可行。In order to evaluate the quality of ginseng preparations, a simple and accurate HPLC method for determining the contents of twenty ginsenosides was established. The results suggested that twenty ginsenosides（ginsenoside Rg1, Re, Rf, Rb1, Rg2, Rc, 20（R）-Rh1, Rb2, Rb3, F1, Rd, Rk3, F2, Rh4, Rg3, protopanaxatriol, compound K, Rg5, Rh2 and protopanaxadiol） were separated at baseline with good linearity（R ≥ 0.999 2）. The method is simple, fast, accurate, and could be applied in the quality control of ginseng preparations.

关 键 词：人参 人参皂苷 高效液相色谱 含量测定 

分 类 号：R917[医药卫生—药物分析学]