一氧化氮间歇熏蒸对木纳格葡萄活性氧代谢的影响  

Effect of Intermittent Fumigation with Nitric Oxide on Reactive Oxygen Species Metabolism of ‘Munage’ Table Grapes

作　　者：张政[1] 王倩[1] 张辉[1] 魏佳[2] 丁娟丽 沈爱燕 吴斌[2,3] ZHANG Zheng WANG Qian ZHANG Hui WEI Jia DING Juanli SHEN Aiyan WU Bin(College of Food Science and Pharmacy, Xinjiang Agricultural University, ürümqi 830052, China Institute of Agro-Products Storage and Processing, Xinjiang Academy of Agricultural Sciences, ürümqi 830091, China Xinjiang Laboratory of Agro-Products Quality and Safety, ürümqi 830091, China)

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052 [2]新疆农业科学院农产品贮藏加工研究所,新疆乌鲁木齐830091 [3]新疆农产品质量安全实验室,新疆乌鲁木齐830091

出　　处：《食品科学》2016年第22期249-254,共6页Food Science

基　　金：国家自然科学基金地区科学基金项目(31560473)

摘　　要：以木纳格葡萄为材料,采用0、300、1 000μL/L一氧化氮(NO)以间歇熏蒸的方式处理木纳格葡萄,每7 d熏蒸1次,共熏蒸8次。测定了不同用量NO熏蒸处理后其活性氧代谢相关酶的变化趋势。比较了NO处理对葡萄果皮以及果肉中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、丙二醛(MDA)含量、超氧阴离子自由基(O_2^-·)生成速率和过氧化氢(H_2O_2)含量变化的差异性,研究NO对葡萄抗氧化酶以及活性氧的影响方式。结果表明:300μL/L NO处理能够提高木纳格葡萄果皮和果肉中SOD、POD、CAT和APX的活性,延缓MDA、O_2^-·和H_2O_2的积累,维持木纳格葡萄果实中活性氧代谢的平衡,抑制膜质过氧化的发生,延缓果实的衰老。The aim of this work was to investigate the impact of nitric oxide（NO） on the metabolism of reactive oxygen species（ROS）. ‘Munage＇ table grapes were treated by intermittent fumigation once every seven days totally 8 times. The effects of NO on the activities of superoxide dismutase（SOD）, peroxidase（POD）, catalase（CAT）, and ascorbate peroxidase（APX） in grape peel and flesh were analyzed. The changes in the contents of malondialdehyde（MDA） and H2O2, as well as superoxide anion radical formation rate were also monitored. The results showed that SOD, POD, CAT and APX activities grape peel and flesh could be improved effectively and the accumulations of MDA, superoxide anion radical and H2O2 were delayed by 300 μL/L NO intermittent fumigation treatment. The NO treatment could maintain the balance of active oxygen metabolism, inhibit membrane lipid peroxidation and delay grape senescence. This study contributed to a comprehensive understanding of the ROS metabolism in ‘Munage’ grape treated with NO and provided useful information for maintaining fruit quality.

关 键 词：一氧化氮 间歇熏蒸 葡萄 活性氧 

分 类 号：TS225.3[轻工技术与工程—粮食、油脂及植物蛋白工程]