程序性死亡配体-1与白细胞介素-10在喉鳞状细胞癌中的表达及意义  

Expression and Role of Programmed death-1 ligand and Interleukin-10 in Laryngeal Squamous Cell Carcinomas

作　　者：叶惠平[1] 梁文卿 李芊芊[1] 张安琪[1] 吴哲丹 喻国冻[1] 

机构地区：[1]贵州医科大学附属医院耳鼻喉科,贵州贵阳550004

出　　处：《贵州医药》2016年第10期1019-1021,共3页Guizhou Medical Journal

基　　金：贵州省科技厅联合基金项目资助(LG-2012-068);贵州医科大学附属医院博士启动基金资助

摘　　要：目的通过检测程序性死亡配体-1(PD-L1)和白细胞介素-10(IL-10)在喉鳞状细胞癌中的表达情况,分析与喉鳞癌的肿瘤部位、病理分级、TNM分期、淋巴结转移等之间的关系。方法应用免疫组化二步法检测30例喉鳞状细胞癌组织(观察组)和15例癌旁正常组织(对照组)中PD-L1及IL-10的表达情况,分析二者与喉鳞状细胞癌临床病理参数的关系及二者表达的相关性。结果 (1)PD-L1在喉鳞状细胞癌组织(56.67%)中的表达明显高于癌旁正常组织(13.33%);喉鳞状细胞癌组织中PD-L1的阳性表达与病理分级、临床分期(TNM分期)、T分期、肿瘤局部淋巴结转移有关(P<0.05),与肿瘤分区、患者年龄无明显相关性。(2)IL-10在喉鳞状细胞癌组织(63.33%)中的表达明显高于癌旁正常组织(20.00%);喉鳞状细胞癌组织中IL-10的阳性表达与TNM分期、T分期、肿瘤局部淋巴结转移有关(P<0.05),与病理分级、肿瘤分区、患者年龄无明显相关性。(3)喉鳞状细胞癌组织中PD-L1、IL-10的表达呈正相关(r=0.522,P<0.05)。结论 (1)PD-L1与IL-10的高表达可能与喉鳞状细胞癌的发病相关,两者参与了肿瘤的发展及转移;(2)免疫卡控点的调控和抑制性细胞因子与肿瘤的免疫逃逸密切相关,PD-L1与IL-10在喉鳞状细胞癌中的表达呈正相关,两者可能协同参与喉鳞状细胞癌的免疫逃逸;(3)联合检测PD-L1与IL-10在喉鳞状细胞癌组织中的表达对喉鳞癌的临床评估有一定价值;为喉鳞癌的免疫治疗提供理论支持。Objective To identify the relationship between the expression of programmed death-1ligand（PD-L1）and interleukin-10（IL-10）and the tumorous division,clinical stage,pathological grade and lymph node metastasis of LSCC.Methods The expression of PD-L1 and IL-10 were detected by two-step immunohistochemistry in thirty cases of LSCC tissues（experiment group）and fifteen cases of para-carcinoma tissues（control group）,the correlation between them and their correlations with the clinicopathological parameters were analyzed.Results The levels of PDL1 positive expression in LSCC tissue（56.67%）were significantly higher than that of histologically normal tissue（13.33%）.In the LSCC tissues,the expression of PD-L1 was significantly associated with the pathological grade,T stage,TNM stage of LSCC and lymph node metastasis（P〈0.05）,but no association was observed with tumor sites and age of patients.The levels of IL-10 expression in LSCC tissue（63.33%）were significantly higher than that of histologically normal tissue（20.00%）.In the LSCC tissues,the expression of PD-L1 was significantly associated with the T stage,TNM stage of LSCC and lymph node metastasis（P〈0.05）,but no association was observed with pathological grade,tumor sites and age of patients.The expression of PD-L1pro-correlated with IL-10 in the LSCC tissues（r=0.522,P〈0.05）.Conclusion The high expression of PD-L1 and IL-10 may be closely related to the occurrence,development and metastasis of LSCC.The expression of PD-L1pro-correlated with IL-10 in the LSCC tissues,they may be interactive with each other in the immune escape of LSCC together.Inhibition of their expression in LSCC might be a new treatment to LSCC.

关 键 词：程序性死亡配体-1 白细胞介素-10 喉鳞状细胞癌 

分 类 号：R739.65[医药卫生—肿瘤]