乳腺癌中HER-2表达与其基因启动子甲基化的关系  

The relationship between HER-2 expression and its methylation of gene promoter in breast cancer

作　　者：王海[1] 凌扬[1] 盛桂凤[1] 刘永萍[2] 周林艳[3] 张亚平[2] 

机构地区：[1]苏州大学附属常州肿瘤医院肿瘤内科,江苏常州213000 [2]苏州大学附属常州肿瘤医院临床肿瘤实验室,江苏常州213000 [3]苏州大学附属常州肿瘤医院病理科,江苏常州213000

出　　处：《中外女性健康研究》2016年第18期1-2,9,共3页Women＇s Health Research

基　　金：江苏省科技厅临床医药专项基金(BL2013012);常州市科技计划项目(CE20155043;CJ20159023)

摘　　要：目的:探讨女性乳腺癌组织中人表皮生长因子受体-2(HER-2)表达与其基因启动子甲基化的关系。方法:应用实时荧光定量PCR方法检测38例乳腺癌组织中HER-2启动子甲基化情况,并结合临床病理资料进行分析。结果:HER-2阴性组的HER-2基因启动子甲基化水平显著高于阳性组(P<0.05)。乳腺癌患者年龄、淋巴结转移、组织学分级及ER与HER-2基因甲基化无明显相关性(P>0.05);乳腺肿瘤大小与HER-2基因甲基化水平呈负相关(r=-0.6036,P<0.05)。结论:HER-2基因甲基化抑制了乳腺癌组织HER-2表达。Objective：To investigate the relationship between the expression of human epidermal growth factor receptor-2（HER-2）and the promoter methylation of HER-2in female breast cancer.Methods：Using real-time fluorescence quantitative PCR method to detect the methylation status of HER-2promoter in 38 cases of breast cancer tissues,and to analyze the clinical pathological data.Results：The methylation level of HER-2gene promoter in HER-2negative group was significantly higher than that in the positive group（P〈0.05）.There was no significant correlation between age,lymph node metastasis,pathological grade,ER and HER-2gene methylation in breast cancer patients（P〉0.05）.Breast tumor size was negatively correlated with methylation level of HER-2gene（r=-0.604,P〈0.05）.Conclusion：Methylation of HER-2gene inhibits the expression of HER-2in breast cancer tissues.

关 键 词：乳腺癌 HER-2 表观遗传学 甲基化 

分 类 号：R737.14[医药卫生—肿瘤]