Wdr5通过Wnt通路促进肝癌细胞增殖  

Wdr5 promoting cells proliferation in hepatocelluar carcinoma through Wnt signaling pathway

作　　者：王晓锋[1,2,3] 王玉平[1,2] 魏文珍 胡晓丹[1,2] 李瑞英[1,2] 周永宁[1,2] 

机构地区：[1]兰州大学第一医院消化科,甘肃兰州730000 [2]兰州大学第一医院甘肃省胃肠病重点实验室,甘肃兰州730000 [3]兰州大学第一医院感染科,甘肃兰州730000

出　　处：《胃肠病学和肝病学杂志》2016年第11期1262-1266,共5页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的研究Wdr5对肝癌细胞增殖的影响及其可能的机制。方法构建Wdr5的shRNA病毒感染肝癌细胞系Huh7和HepG2下调Wdr5的表达,进而通过克隆形成实验和MTT等方法检测下调Wdr5表达后对细胞增殖的影响。结果下调Wdr5后,肝癌细胞系Huh7和HepG2的克隆形成、细胞增殖能力显著降低,Wnt通路关键基因APC和β-catenin显著降低。结论下调Wdr5能抑制肝癌细胞增殖,这一过程可能是通过Wnt通路进行的。Objective To study the effect of Wdr5 on cells proliferation in hepatocellular carcinoma by constructing the Wdr5 knock-out cell models. Methods Two shRNAs targeting Wdr5 were generated： shWdr5 A and shWdr5 B, and then infected Huh7 and HepG2 cells by lentiviral transfeetion. The expression of Wdr5 was detected by qPCR and Western blotting. MTT and colony formation assays were used to observe the effect of Wdr5 on cell proliferation. The expressions of APC and β-catenin were also tested at mRNA and protein level after Wdr5 silencing. Results The expression of Wdr5 in Huh7 and HepG2 cells was significantly down regulated after tansfection. The colony formation and cell proliferation were all inhibited after Wdr5 knockdown. And the expressions of APC and β-catenin were all down-regulated after Wdr5 knockdown. Conclusion Down regulating the expression of Wdr5 could inhibit the cells proliferation of hepatoeellular carcinoma, and this could be involved in the regulation of APC and β-eatenin in Wnt pathway.

关 键 词：Wdr5 肝癌 增殖 WNT通路 APC Β-CATENIN 

分 类 号：R735.7[医药卫生—肿瘤]