丹参酮ⅡA对帕金森病大鼠中脑黑质内磷酸化p38MAPK的影响  

Effects of Tanshinone ⅡA on expression of phosphorylated p38 MAPK in the substantia nigra in Parkinson disease rats

作　　者：徐玉英[1] 彭普基 游言文[2] 任秀花[3] 

机构地区：[1]河南中医学院人体解剖与组织胚胎学教研室,河南郑州450008 [2]郑州大学第一附属医院消化内科,河南郑州450052 [3]郑州大学人体解剖学教研室,河南郑州450001

出　　处：《解剖学研究》2016年第5期355-357,367,共4页Anatomy Research

基　　金：河南中医学院省属科研业务费专项青年项目(2014KYYWF-QN14)

摘　　要：目的研究丹参酮IIA对帕金森病(PD)模型大鼠中脑黑质内酪氨酸羟化酶(TH)和磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)表达的影响,探讨丹参酮ⅡA(TSA)对帕金森病模型大鼠的作用及机制。方法 40只SD雄性大鼠随机分为正常对照组(n=10)、假手术组(sham组;n=10)和模型组(n=20)。模型组又分为生理盐水组(NS组)和处理组(TSA组;n=10)。模型组大鼠采用颈背部皮下注射鱼藤酮制备PD模型;假手术组,在与模型组大鼠相同部位注射等量的不含鱼藤酮的葵花油乳化液;正常组不作处理。模型组在大鼠腹腔内注射生理盐水1ml和丹参酮ⅡA 20 mg/kg,连续注射14 d。免疫组织化学法检测正常对照组、NS组和TSA组大鼠中脑黑质内TH和p-p38MAPK的表达。结果与正常对照组及假手术组比较,模型组大鼠出现典型的PD样症状;与正常对照组比较,生理盐水组大鼠中脑黑质内TH的表达降低,p-p38MAPK的表达增多;与生理盐水组比较,处理组大鼠中脑黑质内TH的表达增多,p-p38MAPK的表达降低,差异均有明显统计学意义。结论丹参酮ⅡA可在一定程度减轻PD模型大鼠多巴胺能神经元的损伤,其作用机制可能与p38MAPK信号转导途径有关。Objective To observe effects of Tanshinone IIA on expression of tyrosine hydroxylase（TH） and phosphorylated p38 mitogen-activated protein kinase（p-p38MAPK） in the substantia nigra in Parkinson disease rats, and to explore the role and mechanism of tanshinone Ⅱ A on Parkinson disease rats. Methods 40 adult SD rats were randomly divided into control group, sham group （n=10） and PD model group （n=20）, PD model group was randomly and evenly divided into two groups： saline group （NS group） and treatment group （TSA group） （n=10）. The PD model group was established by subcutaneous injection of rotenone in the neck and back area, while the injection of sunflower oil emulsion without rotenone at the same point and quantity as the PD model group was applied in the sham group; The control group was not given any intervention. Nature saline lml and tanshinone IIA 20 mg/ kg were given through intraperitoneal injection respectively for 14d in PD model group.TH and p-p38MAPK were measured with im- munohistochemical staining. Results Compared with the control group and sham group, there was typical PD ethology change in PD model group; Compared with the control group, the expression of TH positive neuron decreased, while the expression of p-p38MAPK increased significantly in the substantia nigra in the saline group. Compared with the saline group, the expression of TH positive neu- ron increased, while the expression of p-p38MAPK decreased significantly in the substantia nigra in the TSA group. Conclusion Tanshinone IIA can mitigate the loss of dopaminergic neurons in the PD model rats induced by rotenone, which may be related with p38MAPK signaling pathway.

关 键 词：帕金森病 丹参酮ⅡA 鱼藤酮 P38MAPK 

分 类 号：R742.5[医药卫生—神经病学与精神病学]