PGE2可以恢复缺少内源性PGs的牛体外早期胚胎的正常发育  

PGE2 Can Rescue Normal Development of in vitro Bovine Early Embryos Lacking Endogenous PGs

作　　者：秦国嵩[1,2] 倪和民[1] 刘云海[1] 李贺娟[1] 盛熙晖[1] 齐晓龙[1] 郭勇[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]中国科学院动物研究所,干细胞与生殖生物学国家重点实验室,北京100101

出　　处：《中国农学通报》2016年第32期1-6,共6页Chinese Agricultural Science Bulletin

基　　金：2013年度北京市教委北京市属高等学校创新团队建设与教师职业发展计划项目“体细胞转基因克隆肉牛新品系培育与利用”(No.PXM2013_014207_000067)

摘　　要：旨在研究外源添加PGE2(前列腺素E2)对缺少内源性前列腺素(PGs,Prostaglandins)的牛IVF早期胚胎外发育的影响。在牛IVF胚胎中抑制内源前列腺素合成再外源添加PGE2,以观察PGE2对牛IVF胚胎发育的作用以及起作用的胚胎阶段。并检测PGE2受体的mRNA水平。抑制胚胎内源性前列腺素合成限速酶COX-1和COX-2会使牛IVF胚胎卵裂率、8-16细胞率、囊胚率以及孵化率显著低于常规培养组;而外源添加PGE2能够补偿IVF胚胎因缺少内源性PGs导致的损伤,基本抵消COX-1和COX-2的特异性抑制剂对牛早期胚胎体外发育产生的不利影响;PGE2的添加浓度以1×10-8mol/L为宜,并且其补偿作用发生在8细胞阶段之前。利用半定量RT-PCR未能检测到以上6个阶段IVF胚胎上EP1、EP2、EP3和EP4四种受体的mRNA。PGE2可以恢复缺少内源性PG的牛体外IVF早期胚胎的正常发育。The study aimed to investigate the effect of exogenous prostaglandin vitro development of bovine IVF which lacked endogenous prostaglandins E2 （PGE2） on early embryo in （PGs）. Specific inhibitors of prostaglandins were also added into the cuhure medium of bovine embryos to inhibit the initiation of these embryonic endogenous prostaglandins, and then, an investigation appropriate concentration and work stage of PGE2 on early bovine was conducted to detect the function, the embryos＇ development. In addition, PGE2 specific receptors＇ mRNA level was also respectively detected. It was found that bovine IVF embryonic developmental potentialities could decrease the cleavage rate, 8-16 cell rate, blastocyst rate and hatching blastocyst rate significantly compared with those of the control after those embryos＇ endogenous PGs being thoroughly inhibited. Then, it was also found that adding exogenous PGE2 could rescue the damage embryos lacking endogenous PGE2 affected by the adverse effect of PGE2 specific inhibitors. The PGE2 additive concentration was 1 × 10-8 mol/L, and its compensation effect occurred before 8-cell of IVF suitable stage of those embryos cultured in vitro. However, the mRNA of PGE2＇ s specific receptors： EP1, EP2, EP3 and EP4 were all not detectable by semi-quantitative RT-PCR. PGE2 could rescue the normal development of those in vitro produced bovine early embryos lacking endogenous PGs.

关 键 词：牛 早期胚胎 体外发育 前列腺素合成酶抑制剂 PGE2 

分 类 号：S8[农业科学—畜牧兽医]