南京市春季不同功能区大气微生物群落结构及多样性分析  

Community Structure and Diversity of Airborne Microbes:Different Functional Regions in Spring of Nanjing

作　　者：曲浩丽[1,2] 赵娟[1] 肖永良[1] 曹慧[1] 

机构地区：[1]南京农业大学生命科学学院,农业部农业环境微生物工程重点实验室,南京210095 [2]农业部南京农业机械化研究所,南京210014

出　　处：《中国农学通报》2016年第32期174-181,共8页Chinese Agricultural Science Bulletin

基　　金：江苏省环境监测科研基金项目“南京市不同功能区大气降尘中的微生物多样性研究”(0713)

摘　　要：研究旨在明确南京市不同功能区大气降尘中的微生物多样性分布规律,为控制某些空气传播的微生物病原菌提供理论依据。采用3种空气采样方法对比,通过PCR扩增、基因克隆、RFLP和序列分析对南京市3个不同功能区(NAU,NBG,XWG)的空气降尘进行细菌16S r DNA及真菌18S r DNA分析。结果显示,芽孢杆菌属(Bacillus),假单孢菌属(Pseudomona)及微球菌属(Micrococcus)在南京春季空气细菌中占比较大;而青霉属(Penicillium)、曲霉属(Aspergillus)、链格菌属(Alternaria)及挫孔菌属(Sistotrema)是真菌优势菌属。同时,空气微生物中也存在粘质沙雷氏菌等条件致病菌。该试验确定了春季南京市不同功能区细菌真菌丰度及多样性分布规律,为江苏省乃至全国对空气质量的监测及对大气微生物的研究提供了可行性方法。The study aimed at finding the distribution regularities of atmospheric microbes in differentfunctional regions in Nanjing, thus to provide theoretical evidences for controlling some air-borne pathogenicbacteria. We collected air samples using three methods from three domains（NAU, NBG, XWG） and conductedfurther analysis. Diversity and concentration of bacterial 16 S r DNA and fungal 18 S r DNA in three functionalareas of Nanjing were analyzed by PCR amplification, gene clone, RFLP（restricted fragment lengthpolymorphisms） and sequence analysis. The results showed that air samples derived from the three domainscollected from the spring of Nanjing were dominated by bacteria such as Bacillus, Micrococcus and Pseudomona and Alternaria, Penicillium, Aspergillus and Sistotrema were common fungi as well. Meanwhile,the conditioned pathogens such as serratia marcescens were detected. In general, the abundance and diversitydistribution regularities of bacteria as well as fungi in different domains in spring of Nanjing were confirmed,and the study provided a feasible way for air quality monitoring and atmospheric microorganism research inJiangsu Province and the whole country.

关 键 词：空气细菌 空气真菌 群落结构 微生物多样性 PCR-RFLP 

分 类 号：X172[环境科学与工程—环境科学]