机构地区:[1]浙江大学医学院病理与病理生理学系,浙江杭州310058 [2]宁波市鄞州第二医院病理科,浙江宁波315100
出 处:《浙江大学学报(医学版)》2016年第4期335-341,共7页Journal of Zhejiang University(Medical Sciences)
基 金:浙江省自然科学基金(LY12H16027);国家自然科学基金(30870971)
摘 要:目的:探讨肿瘤相关成纤维细胞( TAF) CD10在结直肠腺瘤癌变和复发中的作用,评价TAF CD10表达在诊断腺瘤癌变中的应用价值。方法:分别收集结直肠低级别腺瘤、高级别腺瘤和结直肠腺癌组织各50例,取配对结直肠腺癌标本远端切缘为正常对照,采用免疫组织化学Envision法测定TAF CD10和肿瘤细胞p53、Ki-67、β-catenin及CyclinD1的表达,分析TAF表达CD10与肿瘤细胞表达p53、Ki-67、βc-atenin 和CyclinD1的相关性。同时收集低级别腺瘤100例,分复发组(43例)和非复发组(57例)两组,其中复发组中复发为结直肠腺癌16例和复发为腺瘤27例,比较CD10在复发组与非复发组间差异及复发为结直肠腺癌与腺瘤间差异。结果:正常黏膜不存在 TAF,其CD10在结直肠低级别腺瘤、高级别腺瘤和腺癌中的阳性率分别为22%、50%和78%,呈逐渐升高趋势(均P <0.05);Ki-67、β-catenin在低级别腺瘤、高级别腺瘤、腺癌中瘤细胞的表达逐渐升高(均P<0.01);CyclinD1在高级别腺瘤中表达高于腺癌和低级别腺瘤(均P >0.05);p53在结直肠腺癌和高级别腺瘤中表达高于低级别腺瘤(均P<00.1);CD10的表达水平与肿瘤细胞p53、Ki-67、β-catenin核阳性表达均呈正相关( r=0.264、0.307、0.320,均P<0.01),而与CyclinD1、β-catenin膜阳性表达无明显相关(r=0.012、-0.073,均 P >0.05)。 CD10在低级别腺瘤复发组表达高于非复发组(P<0.05),在复发为结直肠腺癌组织的表达又高于复发为腺瘤者( P<0.05)。结论:TAF CD 10可能在腺瘤癌变中起重要作用;TAF CD10高表达的腺瘤容易复发且癌变;TAF CD10联合肿瘤细胞p53、Ki-67、β-catenin检测有助于诊断结直肠腺瘤癌变和预测腺瘤复发。O bjective: To investigate the expression of CD10 in tumor-associated fibroblasts ( TAF ) in colorectal adenomas and its relation to cancerization and recurrence of adenoma.Methods: Tissue samples of low-grade adenoma ( n =50 ) , high-grade adenoma (n=50) and colorectal adenocarcinoma (n=50) were collected, and tissue samples at the distal margin of corresponding colorectal lesions were taken as controls.The expression of CD10 in the stromal TAFs, and the expressions ofβ-catenin, Ki-67, p53 and CyclinD1 in tumor cells were detected by immunohistochemistry ( Envision) .The correlation of CD10 expression in stromal TAFs with the expressions of β-catenin, Ki -67, p53 and CyclinD1 in tumor cells was analyzed by Spearmen.One hundred samples of low-grade colorectal adenoma were collected, including 57 non-recurrent cases and 43 recurrent cases ( 16 cases of recurrent adenoma and 27 cases of recurrent adenocarcinoma ); the expression of stromal TAF CD10 were determined and compared among groups.Results: There was no TAF in normal colorectal mucosa.The expression rates of TAF CD10 in low-grade adenoma, high-grade adenoma and colorectal adenocarcinoma were 22%, 50% and 78%, respectively (all P〈0.05).The expression of Ki-67 and β-catenin in low-grade adenoma, high-grade adenoma, colorectal adenocarcinoma was on a rising trend ( all P〈0.01 ) .The expression of CyclinD1 in high-grade adenoma was higher than that in colorectal adenocarcinoma and low-grade adenoma (all P〉0.05).The expression of p53 in colorectal adenocarcinoma and high-grade adenoma was higher than that in low grade adenoma (all P〈0.01).The expression of TAF CD10 was correlated with the expression of p53, Ki-67 and β-catenin-nucleus(r=0.264、0.307、0.320, all P〈0.01) ,but not correlated with CyclinD1 andβ-catenin-membrane (r=0.012、-0.073, all P〉0.05). The TAF CD10 level was significantly higher in low-grade adenoma with recurrence than that in those without recurrence ( P 〈0.05 )
关 键 词:成纤维细胞 白细胞分化抗原10 结直肠肿瘤/病理学 结直肠肿瘤/诊断 腺癌/病理学 腺癌/诊断 肿瘤复发 局部 免疫组织化学
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