BDNF和TrkB在大鼠蛛网膜下腔出血后早期脑损伤中的表达及意义  

Expressions of brain derived neurotrophic factor and tropomyosin-related kinase B and their significances in rats with early brain injury after subarachnoid hemorrhage

作　　者：葛洪良[1] 林元相[1] 林章雅[1] 余良宏[1] 张元隆[1] 康德智[1] 丁陈禹[1] 

机构地区：[1]福建医科大学附属第一医院神经外科,福州350005

出　　处：《中华神经医学杂志》2016年第11期1130-1135,共6页Chinese Journal of Neuromedicine

基　　金：福建省临床重点专科建设项目

摘　　要：目的探讨大鼠蛛网膜下腔出血（SAH）后早期脑损伤（EBI）中脑源性神经营养因子（BDNF）及其酪氨酸激酶受体B（TrkB）的表达情况及其作用。方法将56只雄性SD大鼠按随机数字表法分为假手术组和SAH组（n=28），后者应用颈内动脉线栓穿刺法建立SAH模型。分别于术后24h和72h进行神经功能评分、测定脑组织含水量并应用免疫组化染色和ELISA法检测脑皮质BDNF和TrkB的表达。结果术后24h和72h时，SAH组大鼠的神经功能评分和脑组织含水量均高于假手术组；SAH组大鼠的BDNF免疫组化染色评分[（1．33±0．52）分、（1．67±0．52）分]、表达水平[（12．11±0．44）mg／mL、（15．82±0．89）mg/mL]明显高于假手术组[（0．33±0．52）分、（0．17±0．41）分、（4．92±0．16）mg／mL、（4．93±0．20）mg／mL]，差异均有统计学意义（P〈0．05）；SAH组大鼠的TrkB免疫组化染色评分[（1．17±0．75）分、（2．00±0．00）分]、表达水平[（18．89±0．38）mg／mL、（25．18±0．68）mg／mL]明显高于假手术组[（0．17±0．41）分、（0．33±0．52）分、（8．52±0．41）mg／mL、（8．08±0．34）mg／mL]，差异均有统计学意义（P〈0．05）。结论大鼠SAH后BDNF及TrkB表达明显增加，可能在EBI中发挥着重要的保护作用。Objective To observe the expressions of brain derived neurotrophic factor （BDNF） and tropomyosin-related kinase B （TrkB） in rats with early brain injury （EBI） after subarachnoid hemorrhage, and study the neuroprotective effects of BDNF and TrkB on EBI. Methods Male Sprague-Dawley rats （n=56）, weighing 280-320 g, were randomly divided into sham-operated group and SAH group; SAH models were established by endovascular perforation of internal carotid artery. At 24 and 72 h after modeling, neurological scale scores were recorded; brain water content was measured; immunohistochemical staining and ELISA were used to observe the dynamical expressions of BDNF and TrkB in the brain. Results At 24 and 72 h after modeling, the neurological function scores and brain water content of SAH rats were higher than those of sham-operated group. The expression scores of BDNF in the SAH rats were 1.33±0.52 and 1.67±0.52, and the expression levels were （12.11±0.44） mg/mL and （15.82±0.89） mg/mL; the expression scores of TrkB were 1.17±0.75 and 2.00±0.00, and the expression levels were （18.89±0.38） mg/mL and （25.18±0.68） mg/mL. The expression scores of BDNF in the sham-operated group were 0.33 ±0.52 and 0.17 ±0.41, and the expression levels of BDNF in the sham-operated group was （4.92±0.16） mg/mL and （4.93 ±0.20） mg/mL; the expression scores of TrkB were 0.17±0.41 and 0.33±0.52, and the expression levels were （8.52±0.41） mg/mL and （8.08±0.34） mg/mL. There were significant differences in BDNF and TrkB expressions between the two groups at 24 and 72 h after modeling （P〈0.05）. Conclusion The expressions of BDNF and TrkB increase significantly after SAH, and BDNF and TrkB play protective effect on EBI after SAH.

关 键 词：蛛网膜下腔出血 早期脑损伤 脑源性神经营养因子 酪氨酸激酶受体B 

分 类 号：R743.35[医药卫生—神经病学与精神病学]