Prophylactic effects of alkaloids from Ba lotus seeds on L-NNA-induced hypertension in mice  

作　　者：SUN Peng ZHU Kai WANG Cun LIU Wei-Wei PENG De-Guang ZHAO Xin 

机构地区：[1]Chongqing Collaborative Innovation Center for Functional Food,Chongqing University of Education,Chongqing 400067,China [2]Chongqing Enterprise Engineering Research Center of Ba-lotus Breeding and Deep Processing,Chongqing 400041,China [3]Department of Biological and Chemical Engineering,Chongqing University of Education,Chongqing 400067,China [4]Chongqing Engineering Research Center of Functional Food,Chongqing University of Education,Chongqing 400067,China [5]Chongqing Engineering Laboratory for Research and Development of Functional Food,Chongqing University of Education,Chongqing 400067,China [6]School of Public Health and Management,Chongqing Medical University,Chongqing 400016,China

出　　处：《Chinese Journal of Natural Medicines》2016年第11期835-843,共9页中国天然药物（英文版）

基　　金：supported by Chongqing Engineering Research Center for Functional Food(No.cstc2015yfpt_gcjsyjzx0027);the Program for Innovation and Team Building at the Chongqing Institute of Higher Education(No.CXTDX201601040)

摘　　要：Alkaloids from Ba lotus seeds(ABLS) are a kind of important functional compounds in lotus seeds. The present study was designed to determine its hypertension prophylactic effects in the L-NNA-induced mouse hypertension model. The mice were treated with ABLS, the serum and tissues levels of NO, MDA, ET-1, VEGF, and CGRP were determined using the experimental kits, the mRNA levels of various genes in the heart muscle and blood vessel tissues were further determined by RT-PCR assay. ABLS could reduce the systolic blood pressure(SBP), mean blood pressure(MBP), and diastolic blood pressure(DBP), compared to that of the model control group. After ABLS treatment, the NO(nitric oxide) contents in serum, heart, liver, kidney and stomach of the mice were higher than that of the control mice, but the MDA(malonaldehyde) contents were lower than that of the control mice. The serum levels of ET-1(endothelin-1), VEGF(vascular endothelial growth factor) were decreased after ABLS treatment, but CGRP(calcium gene related peptide) level was increased. The ABLS treated mice had higher mRNA expressions of HO-1, nNOS, and eNOS and lower expressions of ADM, RAMP2, IL-1β, TNF-α, and iNOS than the control mice. Higher concentration of ABLS had greater prophylactic effects, which were close to that of the hypertension drug captopril. These results indicated the hypertension prophylactic effects of ABLS could be further explored as novel medicine or functional food in the future.Alkaloids from Ba lotus seeds （ABLS） are a kind of important functional compounds in lotus seeds. The present study was designed to determine its hypertension prophylactic effects in the L-NNA-induced mouse hypertension model. The mice were treated with ABLS, the serum and tissues levels of NO, MDA, ET-1, VEGF, and CGRP were determined using the experimental kits, the mRNA levels of various genes in the heart muscle and blood vessel tissues were further determined by RT-PCR assay. ABLS could reduce the systolic blood pressure （SBP）, mean blood pressure （MBP）, and diastolic blood pressure （DBP）, compared to that of the model control group. After ABLS treatment, the NO （nitric oxide） contents in serum, heart, liver, kidney and stomach of the mice were higher than that of the control mice, but the MDA （malonaldehyde） contents were lower than that of the control mice. The serum levels of ET-1 endothelin-l）, VEGF （vascular endothelial growth factor） were decreased after ABLS treatment, but CGRP （calcium gene related peptide） level was increased. The ABLS treated mice had higher mRNA expressions of riO-l, nNOS, and eNOS and lower expressions of ADM, RAMP2, IL-lfl, TNF-a, and iNOS than the control mice. Higher concentration of ABLS had greater prophylactic effects, which were close to that of the hypertension drug captopril. These results indicated the hypertension prophylactic effects of ABLS could be further explored as novel medicine or functional food in the future.

关 键 词：Ba lotus seed HYPERTENSION ALKALOIDS L-NNA MICE 

分 类 号：R965[医药卫生—药理学]