Cebpa is essential for the embryonic myeloid progenitor and neutrophil maintenance in zebrafish  

作　　者：Yimei Dai Lu Zhu Zhibin Huang Minyu Zhou Wan Jin Wei Liu Mengchang Xu Tao Yu Yiyue Zhang Zilong Wen Wangjun Liao Wenqing Zhang 

机构地区：[1]Division of Life Science, State Key Laboratory of Molecular Neuroscience, and Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology [2]Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology,School of Basic Medical Sciences, Southern Medical University [3]Department of Oncology, Nanfang Hospital, Southern Medical University

出　　处：《Journal of Genetics and Genomics》2016年第10期593-600,共8页遗传学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 31271564, 31229003 and 31271574);the Team Program of Guangdong Natural Science Foundation (No. 2014A030312002);the Research Grants Council of the HKSAR (Nos. 663212, HKUST5/CRF/12R and AoE /M-09/12)

摘　　要：In vertebrates, myeloid cells arise from multiple waves of development： the first or embryonic wave of myelopoiesis initiates early from non-hematopoietic stem cell（HSC） precursors and gives rise to myeloid cells transiently during early development; whereas the second or adult wave of myelopoiesis emerges later from HSCs and produces myeloid cells continually during fetal and adult life. In the past decades, a great deal has been learnt about the development of myeloid cells from adult myelopoiesis, yet the genetic network governing embryonic myelopoiesis remains poorly defined. In this report, we present an in vivo study to delineate the role of Cebpa during zebrafish embryonic myelopoiesis. We show that embryonic myelopoiesis in cebpa-deficient zebrafish mutants initiates properly but fails to produce macrophages and neutrophils. The lack of macrophages and neutrophils in the mutants is largely attributed to the cell cycle arrest of embryonic myeloid progenitors, resulting in the impairment of their maintenance and subsequent differentiation. We further show that Cebpa, perhaps acting cooperatively with Runx1, plays a critical role in embryonic neutrophil maintenance. Our findings reveal a new role of Cebpa in embryonic myelopoiesis.In vertebrates, myeloid cells arise from multiple waves of development： the first or embryonic wave of myelopoiesis initiates early from non-hematopoietic stem cell（HSC） precursors and gives rise to myeloid cells transiently during early development; whereas the second or adult wave of myelopoiesis emerges later from HSCs and produces myeloid cells continually during fetal and adult life. In the past decades, a great deal has been learnt about the development of myeloid cells from adult myelopoiesis, yet the genetic network governing embryonic myelopoiesis remains poorly defined. In this report, we present an in vivo study to delineate the role of Cebpa during zebrafish embryonic myelopoiesis. We show that embryonic myelopoiesis in cebpa-deficient zebrafish mutants initiates properly but fails to produce macrophages and neutrophils. The lack of macrophages and neutrophils in the mutants is largely attributed to the cell cycle arrest of embryonic myeloid progenitors, resulting in the impairment of their maintenance and subsequent differentiation. We further show that Cebpa, perhaps acting cooperatively with Runx1, plays a critical role in embryonic neutrophil maintenance. Our findings reveal a new role of Cebpa in embryonic myelopoiesis.

关 键 词：CEBPA Embryonic myelopoiesis Development ZEBRAFISH 

分 类 号：Q954.4[生物学—动物学]