靶向ERR α siRNA对不同类型子宫内膜癌细胞凋亡的影响  

The influence of apoptosis in different types of endometrial cancer cells by siRNA targeting on ERR α

作　　者：毛晓丹[1] 孙蓬明[1] 林东红[2] 阮冠宇[1] 董滨华[1] 林芬[1] MAO Xiaodan SUN Pengming LIN Donghong RUAN Guanyu DONG Binhua LIN Fen(Fujian Provincial Maternal and Children Hospital of Fujian Medical University, Fuzhou 350001, China)

机构地区：[1]福建医科大学附属福建省妇幼保健院妇科肿瘤实验室,350001 [2]福建医科大学医学与技术工程学院

出　　处：《中国妇产科临床杂志》2016年第6期538-541,共4页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：福建省科技厅重点项目(2008I0011);福建省卫计委青年科研课题(2015-1-16);福建省卫计委青年科研课题(2014-2-5)

摘　　要：目的探究si RNA作用于不同类型子宫内膜癌细胞雌激素受体相关受体α(estrogen receptor-related receptorα,ERRα)的表达及细胞的凋亡情况。方法 si RNA处理RL952、AN3-CA、HEC-1A和HEC-1B细胞,以RL952、AN3-CA为Ⅰ型子宫内膜癌体外模型,HEC-1A、HEC-1B为Ⅱ型子宫内膜癌体外模型;采用荧光定量PCR法检测ERRαm RNA的表达,Western blot法检测ERRα蛋白的表达,流式细胞仪分别检测si RNA干扰后4株子宫内膜癌细胞的凋亡情况。结果 si RNA组较对照组ERRαm RNA表达水平显著下降(RL952:0.701±0.017 vs.1.165±0.019;AN3-CA:0.899±0.019 vs.1.362±0.007;HEC-1A:0.093±0.004 vs.0.541±0.038;HEC-1B:0.158±0.004 vs.0.356±0.001;P均<0.05)。各株细胞si RNA组与对照组ERRαm RNA的表达比较,差异有统计学意义(P<0.05),ERRαm RNA表达分别减少了39.77%、34.01%、82.82%和55.78%。West ern blot结果显示,4株细胞RNA干扰后ERRα蛋白表达与m RNA表达趋势一致。4株细胞凋亡率:RL952为(30.000±1.056)%vs.(10.572±1.027)%;AN3-CA为(32.931±1.613)%vs.(10.274±0.882)%;HEC-1A为(17.272±0.950)%vs.(8.704±0.608)%;HEC-1B为(18.703±1.877)%vs.(3.801±0.265)%,si RNA干扰4株细胞后凋亡率与各自对照组比较,差异均有统计学意义(P<0.05)。结论 si RNA能够有效抑制子宫内膜癌中ERRα的表达,ERRα低表达可能与子宫内膜癌细胞的凋亡增加密切相关。Objective To explore the expression of estrogen receptor-related receptor α （ ERR α ） mRNA interfered with siRNA and the apoptosis of different types endometrial cancer cells .Methods Detected RL952,AN3- CA,HEC-1A and HEC-1B cells mRNA and protein expression of ERR α by fluorescence quantitative PCR and Western blot respectively after siRNA. Then, the changes of cell apoptosis were measured by FCM. Results The mRNA expressions of ERR α in siRNA groups were significantly decreased than the control group, which were RL952 （0.701±0.017 vs 1.165 ±0.019）, AN3-CA （0.899±0.019 vs 1.362±0.007）, HEC-1A （0.093±0.004 vs 0.541±0.038） and HEC-1B （0.158±0.004 vs 0.356±0.001）, respectively （P 〈 0.05） . While the expression levels of ERR α reduced 39.77%, 34.01% , 82.82% and 55.78% ,respectively. A similar result was observed in the protein expression of ERR α. FCM described the ratios of cell apoptosis in each cell line： RL952 （30.000±1.056）% vs （10.572±1.027）%,AN3-CA （32.931±1.613）% vs （10.274±0.882）%,HEC-1A （17.272±0.949）% vs （8.704±0.608）%,HEC-1B （18.703±1.877）% vs （3.801±0.265）%.The apoptosis ratio of CON group was outstanding lower than KD group in each cell （P 〈 0.05） . Conclusions siRNA can targeting inhibited the expression of ERR α in different types endometrial cancer cells; ERR α low expressing had a close relationship with the increasing cell apoptosis in endometrial cancer ceils.

关 键 词：雌激素受体相关受体α SIRNA 凋亡 子宫内膜癌细胞 

分 类 号：R737.33[医药卫生—肿瘤]