Wnt诱导分泌蛋白-1在PHC中的表达及意义  

Expression of Wnt induced secreted protein-1 in primary hepatic carcinoma and its clinical significance

作　　者：陈晟[1] 简易成[1] 周玮[2] 过欣来[2] 郑亚新[3] 熊伍军[1] CHEN Sheng JIAN Yi-cheng ZHOU Wei GUO Xin-lai ZHENG Ya-xin XIONG Wu-jun(Dept. of Gastroenterology, East Hospital, Tongji University, Shanghai 200120, China Dept. of General Surgery, East Hospital, Tongji University, Shanghai 200120, China Dept. of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China)

机构地区：[1]同济大学附属东方医院消化内科,上海200120 [2]同济大学附属东方医院普外科,上海200120 [3]第二军医大学附属东方肝胆医院肝脏外科,上海200438

出　　处：《同济大学学报（医学版）》2016年第5期29-33,共5页Journal of Tongji University(Medical Science)

基　　金：上海市浦东新区肝脏病专科区重点专科基金(PWZz2013-05)

摘　　要：目的探讨Wnt诱导分泌蛋白-1(Wnt induced secreted protein-1,WISP-1)在原发性肝癌(primary hepatic carcinoma,PHC)中表达水平变化及其意义。方法收集34例PHC患者及12例肝血管瘤患者手术组织标本(包括肿瘤组织及其相应的癌旁/瘤旁组织),用RT-q PCR方法检测各组WISP-1基因表达水平,用Western印迹法测定WISP-1蛋白表达水平,分析WISP-1表达与PHC患者临床特征的相关性。结果 WISP-1基因在70.6%(24/34)的PHC患者中高表达,肝癌组织中WISP-1的平均表达水平是正常肝组织的(2.65±1.16)倍,差异有统计学意义(P=0.011);Western印迹法测定的蛋白表达水平与基因表达水平一致。WISP-1基因表达与肝癌的病理分级及肿瘤分期有明显相关性,而与年龄、性别、AFP水平、肝癌类型及肿瘤位置无明显相关性。结论 WISP-1在PHC中高表达,并与其病程进展相关,WISP-1可能是治疗PHC的潜在靶点。Objective To investigate the expression of Wnt induced secreted protein-1 （ WISP-1 ） in primary hepatic carcinoma（PHC） and its clinical significance. Methods The expression of WISP-1 mRNA and protein in 34 PHC specimens and matched normal liver tissues was detected by quantitative RT-qPCR assay and Western blot, respectively. The correlation between the expression level and clinical features was analyzed. Results WISP-1 gene overexpression was found in 70.6% （24/34） hepatic cancers, and the average expression level of WISP-1 in HCC tissues was 2.65 ＋ 1. 16 times higher than that in normal liver tissues （ P = 0.011 ）. The WISP-1 protein expression was basically in accordance with mRNA expression. The expression of WISP-lmRNA was correlated with pathologic grade and tumor stage, but no with age, sex, AFP level, histological type and tumor site. Conclusion Our results suggest that WISP-1 might be involved in the development and progression of PHC and may serve as a potential target for therapeutic intervention of PHC.

关 键 词：Wnt诱导分泌蛋白-1 原发性肝肿瘤 临床意义 

分 类 号：R735.7[医药卫生—肿瘤]