印记基因CDKN1C在胎盘中的表达及其与适于和小于胎龄儿出生体重的关系  

Placental expression of CDKN1C imprinted gene and birth weight of appropriate and small for gestational age neonates

作　　者：刘慧强[1] 邢燕[1] 崔蕴璞[1] 石丹丹[1] 孙艳[1] 王新利[1] Liu Huiqiang Xing Yan Cui Yunpu Shi Dandan Sun Yan Wang Xinli(Department of Pediatrics, Peking University Third Hospital, Beijing 100191, China)

机构地区：[1]北京大学第三医院儿科,100191

出　　处：《中华围产医学杂志》2016年第11期867-871,共5页Chinese Journal of Perinatal Medicine

摘　　要：目的 探讨胎盘组织中印记基因CDKN1C的表达情况及其与适于胎龄儿（appropriate for gestational age，AGA）和小于胎龄儿（small for gestational age，SGA）出生体重的关系。方法 收集2014年1月1日至12月31日于北京大学第三医院分娩的足月SGA共29例，按照1︰1的比例选择出生胎龄相差不超过1周的AGA为对照组。胎盘娩出后迅速留取胎盘组织，分别采用实时荧光定量-聚合酶链反应和Western印迹技术测定胎盘组织CDKN1C的mRNA和蛋白表达量。采用两独立样本t检验、χ2检验和Pearson相关分析进行统计学处理。结果 SGA组胎盘组织中CDKN1C mRNA表达水平显著高于AGA组（0.133±0.059与0.100±0.046，t=2.401，P=0.020）；蛋白表达量也明显高于AGA组，差异有统计学意义（0.280±0.043与0.190±0.041，t=8.410，P=0.000）。2组胎盘组织CDKN1C mRNA表达量均与出生体重负相关（SGA组r=－0.587，P=0.001；AGA组r=－0.569，P=0.001），SGA组的相关性（r2=0.344）稍强于AGA组（r2=0.324）。2组胎盘组织CDKN1C蛋白表达量亦与出生体重呈负相关（SGA组r=－0.579，P=0.001；AGA组r=－0.497，P=0.006），SGA组的相关性（r2=0.335）稍强于AGA组（r2=0.247）。胎盘组织CDKN1C mRNA表达量与性别相关的出生体重呈负相关，与男婴的相关性（r2=0.293）略强于女婴（r2=0.185）；胎盘组织CDKN1C蛋白表达量与性别相关的出生体重呈负相关，与男婴的相关性（r2=0.730）强于女婴（r2=0.601）。胎盘组织CDKN1C mRNA表达量与胎盘重量相关性无统计学意义（SGA组r=0.119，P=0.540；AGA组r=－0.069，P=0.722），CDKN1C蛋白表达量与胎盘重量间相关性亦无统计学意义（SGA组r=0.126，P=0.515；AGA组r=－0.247，P=0.196）。结论 印记基因CDKN1C在胎盘组织的表达量可能与足月SGA出生体重有关，尤其是男婴。Objective To investigate the relationship between the expression of imprinted gene CDKN1C in placenta and the birth weight of neonates.Methods Twenty-nine term small for gestational age （SGA） neonates admitted to Peking University Third Hospital from January 1, 2014 to December 31, 2014 were recruited, and 29 appropriate for gestational age （AGA） neonates with a difference of not more than one week in gestational age served as controls. Fresh placental tissue was collected and the expression of imprinted gene CDKN1C mRNA in the placenta were detected by real-time fluorescence quantitative-polymerase chain reaction, and its protein expression was estimated by Western-blot. Chi-square test, independent-sample t test, Pearson＇s correlation analysis were used for statistical analysis.Results The CDKN1C mRNA expression level in SGA was significantly higher than that in AGA （0.133±0.059 vs 0.100±0.046, t=2.401, P=0.020）, so was the CDKN1C protein expression （0.280±0.043 vs 0.190±0.041, t=8.410, P=0.000）. The CDKN1C mRNA expression levels were negatively correlated with birth weight in both groups （SGA group, r=-0.587, P=0.001; AGA group, r=-0.569, P=0.001）, and the correlation was slightly stronger in SGA （r2=0.344） than in AGA （r2=0.324）. The CDKN1C protein expression levels of the two groups were negatively correlated with birth weight （SGA group, r=-0.579, P=0.001; AGA group, r=-0.497, P=0.006）, the correlation being stronger in SGA group （r2=0.335） than in AGA group （r2=0.247）. The CDKN1C mRNA and protein expression levels of the two groups were negatively correlated with birth weight for gender, especially in males [mRNA： r2=0.293（male） vs r2=0.185（female）; protein： r2=0.730 （male） vs r2=0.601（female）]. Neither CDKN1C mRNA nor protein expression level was correlated to the placenta weight （mRNA： SGA group, r=0.119, P=0.540; AGA group, r=-0.069, P=0.722; protein： SGA group, r=0.126, P=0.515; AGA group, r=-0.247, P=0.196）.Conclusions The expre

关 键 词：基因组印迹 胎盘 周期素依赖激酶抑制剂p57 出生体重 婴儿 新生 

分 类 号：R714.5[医药卫生—妇产科学]