米氏凯伦藻的培养条件及其抑制α-葡萄糖苷酶和血管紧张素I-转换酶活性的研究  

Study on the culture conditions of Karenia mikimotoi and its inhibitory activity onα-glucosidase and ACE

作　　者：陈宇婷[1] 胡晓娟[1] 赵莹[1] 李敏晶[1] 张敏[1] 李伟[2] 谭成玉[1] CHEN Yu-ting HU Xiao-juan ZHAO ging LI Min-jing ZHANG Min L TAN Cheng-yu(College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China College of food science and engineering, Dalian Ocean University, Dalian 116023, China)

机构地区：[1]大连海洋大学海洋科技与环境学院,辽宁大连116023 [2]大连海洋大学食品科学与工程学院,辽宁大连116023

出　　处：《精细与专用化学品》2016年第11期20-23,共4页Fine and Specialty Chemicals

基　　金：国家自然科学基金项目(41306137);辽宁省自然科学基金项目(2013020127);辽宁省高校杰出青年学者成长计划(LJQ 2014078)

摘　　要：通过对米氏凯伦藻实验室培养条件中初始pH值、接种量、培养温度、光照等因素进行研究,探讨米氏凯伦藻的适宜培养条件,并对培养的米氏凯伦藻提取物进行抑制α-葡萄糖苷酶和血管紧张素I-转换酶(ACE)活性实验。结果表明,米氏凯伦藻的适宜培养条件为:初始pH值为7,接种量为100∶10,培养温度为30℃/25℃(昼/夜),光照强度为3500Lx。其水提物在浓度为8mg·mL^(-1)时对α-葡萄糖苷酶的抑制率为34.1%,在浓度为100mg·mL^(-1)时对ACE的抑制率为19.7%,具有一定的抑制活性。The culture conditions of Karenia mikimotoi were studied such as initial pH,inoculation amount,culture temperature and intensity of illumination.The optimum conditions were as follows：initial pH was 7,inoculation amount was 100∶10,culture temperature was 30℃/25℃（day/night）and the intensity of illumination was3500 Lx.In addition,the inhibitory activity onα-glucosidase of the water extract at the concentration of 8 mg·mL-（-1） has inhibitory rate of 34.1%,and the inhibitory rate of the water extract on angiotensin I-converting enzyme（ACE）were 19.7% at the concentration of 100mg·mL-（-1）,which showed that the water extract from the Karenia mikimotoi had some inhibitory activity.

关 键 词：米氏凯伦藻 培养条件 Α-葡萄糖苷酶 ACE 

分 类 号：Q949.2[生物学—植物学]