MMP-9基因敲除小鼠磨牙牙胚中釉原蛋白和成釉蛋白表达的研究  

Expression of Amelogenin and Ameloblastin in the Molar Tooth Germs of MMP-9 Knock-out Mice

作　　者：李桐[1] 袁国华[1] 聂敏[1] 杨国斌[1] 

机构地区：[1]武汉大学口腔医学院口腔生物医学工程教育部重点实验室,湖北武汉430079

出　　处：《口腔医学研究》2016年第11期1117-1121,共5页Journal of Oral Science Research

基　　金：国家自然科学基金资助项目(编号:81371105;81570942)

摘　　要：目的:检测MMP-9基因敲除小鼠(MMP-9^(-/-))和野生型小鼠磨牙牙胚中釉原蛋白和成釉蛋白的表达。方法:使用免疫组化方法检测出生后2d(P2)、5d(P5)、7d(P7)野生型小鼠和MMP-9^(-/-)小鼠下颌磨牙牙胚中釉原蛋白和成釉蛋白的表达分布。结果:在P2时,釉原蛋白主要表达在成熟的成釉细胞胞、及其相邻的成牙本质细胞内,敲除MMP-9后,釉原蛋白的表达可扩散至牙乳头间充质;在P5和P7时,釉原蛋白的表达分布在野生型小鼠和MMP-9^(-/-)小鼠中无差异,但在MMP-9^(-/-)小鼠中釉原蛋白在成釉细胞中的表达量明显增强。在P2、P5和P7时,成釉蛋白的表达分布和表达量在野生型小鼠和MMP-9^(-/-)小鼠中均无差异。结论:MMP-9可能通过酶切釉原蛋白而影响其表达分布。Objective：To detect the expressions of Amelogenin and Ameloblastin in wild-type mice and MMP-9gene knock-out mice（MMP-9-（-/-））.Methods：The expression levels of Amelogenin and Ameloblastin in tooth germs were detected with immunohistochemistry in wild-type and MMP-9-（-/-）mice at post-natal day 2（P2）,5（P5）,and 7（P7）.Results：At P2,Amelogenin was mainly expressed in matured ameloblasts and the underlying odontoblasts.However,in MMP-9-（-/-）mice,the expression of amelogenin was expanded to the dental mesenchyme.No difference was found in the expression pattern of Amelogenin between wild-type mice and MMP-9-（-/-）mice at P5 and P7.However,the expression level of Amelogenin in ameloblasts was highly increased in MMP-9-（-/-）mice compared with that in wild-type mice.No obvious differences were detected in the expression pattern or expression level of Ameloblastin between wild-type mice and MMP-9-（-/-）mice at P2,P5,and P7.Conclusion：MMP-9might be able to degrade Amelogenin,and further influence the expression pattern of Amelogenin.

关 键 词：釉原蛋白 成釉蛋白 磨牙牙胚 基质金属蛋白酶9 

分 类 号：R781[医药卫生—口腔医学]