野黄芩素协同ABT-737抗头颈鳞癌作用的研究  

Synergistic Anti-tumor Activity of Scutellarein and ABT-737 on Head and Neck Squamous Cell Carcinoma

作　　者：静广平[1] 吕克文[1] 张晗[1] 吕行[1] 

机构地区：[1]哈尔滨医科大学附属口腔医院颌面外科,黑龙江哈尔滨150001

出　　处：《口腔医学研究》2016年第11期1188-1192,共5页Journal of Oral Science Research

基　　金：黑龙江省教育厅科学技术研究项目(编号:12541294)

摘　　要：目的:研究野黄芩素协同ABT-737在体外对人头颈鳞癌HN30细胞的凋亡诱导作用,探讨其可能的作用机制。方法:MTT法检测野黄芩素协同ABT-737抑制细胞增殖作用;流式细胞术检测细胞凋亡;JC-1染色法检测线粒体膜电位变化;western blotting检测细胞凋亡蛋白的表达;利用SiRNA技术沉默基因表达。结果:野黄芩素协同ABT-737在体外对人头颈鳞癌HN30细胞增殖有抑制作用,且呈量效关系;两者可协同诱导HN30细胞凋亡;使线粒体膜电位去极化;下调抗凋亡蛋白Bcl-2、Bcl-XL表达,上调Bax表达;SiRNA抑制Mcl-1表达可以显著增强野黄芩素和ABT-737协同诱导HN30细胞凋亡的作用。结论:野黄芩素在体外可以协同ABT-737诱导人头颈鳞癌HN30细胞凋亡,野黄芩素下调Mcl-1蛋白的表达,抑制Mcl-1对ABT-737抗肿瘤作用的影响,是两者协同作用的机制。Objective：To investigate the synergistic anti-tumor activity of Scutellarein and ABT-737 on head and neck squamous cell carcinoma HN30 cell line in vitro and to discuss the mechanisms.Methods：Inhibitory effects of ABT-737 and Scutellarein on HN30 cells were detected by MTT assay.Cell apoptosis was analyzed by flow cytometry.Mitochondrial transmembrane potential（ΔΨm）was analyzed by JC-1staining.The expression of the protein related to apoptosis was quantified by western blot.Genes silencing was conducted by SiRNA.Results：Scutellarein and ABT-737 could synergistically inhibit the proliferation of HN30 cells in vitro in a dose-dependent manner.Scutellarein and ABT-737 could synergistically induce the apoptosis of HN30 cells,induce the Mitochondrial membrane potential depolarization,down-regulate the expression of Bcl-2and Bcl-XL,and up-regulate the expression Bax.Inhibition of the expression of Mcl-1induced by SiRNA could significantly enhance the synergistic effect of scutellarein and ABT-737 on HN30cells apoptosis.Conclusion：Scutellarein and ABT-737 could synergistically induce the apoptosis of HN30 cells in vitro.The expression of Mcl-1was down regulated by scutellarein and was correlated with the drug resistance for ABT-737.

关 键 词：野黄芩素 ABT-737 头颈鳞癌 协同作用 

分 类 号：R739.91[医药卫生—肿瘤]