响应面法优化近江牡蛎多糖提取工艺  

Optimization for extraction process of polysaccharide from Ostrea rivularis Gould by response surface methodology

作　　者：牛改改 游刚[1,2,3] 张晨晓[1,2,3] 余振宁[1] 

机构地区：[1]钦州学院食品工程学院,广西钦州535000 [2]广西北部湾海洋生物多样性养护重点实验室/北部湾海洋生物资源开发与保护重点实验室,广西钦州535000 [3]广西北部湾特色海产品资源开发与高值化利用高校重点实验室,广西钦州535000

出　　处：《南方农业学报》2016年第6期994-1000,共7页Journal of Southern Agriculture

基　　金：广西北部湾海洋生物多样性养护重点实验室项目(2015ZC09);北部湾海洋生物资源开发与保护重点实验室项目(2015ZC15)

摘　　要：【目的】优化近江牡蛎多糖提取工艺,为不同海域牡蛎多糖含量对比及其结构分析打下基础。【方法】以近江牡蛎为原料,通过单因素试验考察液料比、提取温度、提取时间、提取次数4个因素对其多糖提取率的影响,并在此基础上选取液料比、提取时间及提取温度3个因素为变量,近江牡蛎多糖提取率为考察指标,采用Box-Behnken试验设计方法建立回归模型,以优化近江牡蛎多糖提取工艺条件。【结果】通过响应面法建立近江牡蛎多糖提取率(Y)与液料比(A)、提取时间(B)、提取温度(C)的二次多项式数学模型为:Y=9.65-0.18A-0.071B+0.080C-0.27AB-0.24AC-0.52BC-2.6A2-1.34B2-1.35C2,该模型拟合度好;最佳提取工艺条件为:液料比90∶1、提取温度90℃、提取时间3 h、提取次数1次,在此条件下的近江牡蛎多糖提取率为9.72%,与预测值(9.66%)相对偏差为0.62%。【结论】采用响应面分析法优化近江牡蛎多糖提取工艺具有可行性,可用于实际生产,以提高多糖提取率。[Objective]The present experiment was conducted to optimize the extraction process of polysaccharide from Ostrea rivularis Gould, in order to provide a theoretical basis for comparing and analyzing content and structure of oyster polysaecharide in different sea areas. [Method]The effects of liquid-solid ratio, extraction temperature, extraction time and extraction times on extraction rate of polysaccharides from O. rivularis were investigated by single-factor test. Mean- while, based on the results of single-factor test, liquid-solid ratio, extraction time and extraction temperature was chosen as independent variables, and the extraction rate of oyster polysaccharide was selected as response value. Then the regres- sion model was established by Box-Behnken experiment design method, so as to optimize extraction process of polysaccha- ride from O. rivular/s. [Result]The mathematical model for quadratic polynomial of polysaccharide extraction rate （Y） with liquid-solid ratio （A）, extraction time （B）, extraction temperature （C） was established by response surface method, as fol- low： Y=9.65-0.18A-0.071B＋0.080C-0.27AB-0.24AC-0.52BC-2.6A2-1.34B2-1.35C2, with high fitting degree. The opti- mum conditions of extraction process were as follows： 90：1 of liquid-solid ratio, 90℃ of extraction temperature, 3 h of ex- traction time, extracting 1 time. Under the above optimum conditions, the extraction rate of polysaccharide from O. rivularis reached up to 9.72%, which was close to the predicted value（9.66%）, with relative deviation of 0.62%. [Conclusion]The response surface methodology is a workable method to optimize extraction conditions of polysaecharide from O. rivularis, which should be applied to increase effectively extraction rate of polysaceharides in actual production.

关 键 词：近江牡蛎 多糖 响应面分析 提取工艺优化 

分 类 号：R284.2[医药卫生—中药学]