漆酶-TEMPO体系选择性氧化壳聚糖C-6伯羟基提高其抗氧化性能的研究  

Study on the selective oxidation of the C-6-hydroxyl of chitosan by laccase-TEMPO system to improve antioxidant capability of the product

作　　者：裴继诚[1] 殷允北 胡稳 卜鑫[1] 申正会[1] 张方东[1] 

机构地区：[1]天津科技大学造纸学院,天津市制浆造纸重点实验室,天津300457

出　　处：《功能材料》2016年第6期10-15,共6页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(31170548);国家级大学生创新创业训练计划资助项目(201510057099)

摘　　要：利用漆酶-TEMPO体系选择性地氧化壳聚糖C-6伯羟基,对氧化产物分别进行红外光谱、13C NMR、离子色谱和热失重分析,确定产物结构的变化,并进一步对其抗氧化性进行研究。结果表明,经漆酶-TEMPO体系氧化后,壳聚糖分子结构中的C-6伯羟基被选择性地氧化成羧基,其它结构基本不变,其产物为氧化壳聚糖钠盐;壳聚糖氧化产物与原样相比,热稳定性得到了提高;壳聚糖经漆酶-TEMPO体系氧化后的ABTS自由基清除率5min后达到66.36%,抗氧化性能得到改善。Research adopting laccase-TEMPO system to selectively oxidise the C-6-hydroxyl of chitosan,the FT-IR,^13 C NMR,Na^＋ chromatogram and TGA were used to characterize oxidation products,and further to study its antioxidant capability.Results showed that the C-6-hydroxyl of chitosan were selectively oxidised to carbox-yl,and other basic structure unchanged,the product is sodium salt of oxidised chitosan;compared to the oxida-tion product of chitosan with the original,can improve the thermal stability;after standing for 5 min,the oxi-dation product of chitosan provided an ABTS·＋ radical scavenging ability of 66.36%,indicate that antioxidant capability of the oxidation product was improved.

关 键 词：漆酶 TEMPO 壳聚糖 抗氧化性 

分 类 号：TB332[一般工业技术—材料科学与工程]