侧孢短芽孢杆菌抗菌肽的结构与性质  

Structure and Function Characterization of Antimicrobial Peptide from Brevibacillus laterosporus

作　　者：马俊美[1] 宁亚维[1] 王志新[1] 孙国杰[1] 李兴峰[1] 贾英民[1] MA Junmei NING Yawei WANG Zhixin SUN Guojie LI Xinjeng JIA Yingmin(School of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018, China)

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018

出　　处：《食品与生物技术学报》2016年第6期629-634,共6页Journal of Food Science and Biotechnology

基　　金：国家"十二五"科技支撑计划项目(2013BAD10B03);国家农业科技成果转化项目(2013GB2A200032);河北省拔尖人才计划项目(098293)

摘　　要：为了研究侧孢短芽孢杆菌S62-9所产抗菌肽的结构与性质,采用MALDI-TOF/TOF、FT-IR对抗菌肽的相对分子质量、氨基酸序列及二级结构进行解析。结果显示,该抗菌肽的相对分子质量为1602.0044 Da,由11个氨基酸组成：L-Y-K-L-V-K-V-V-L-N-M,其N端有一分子胆碱修饰,C端有细交链孢菌酮酸衍生物修饰,二级结构主要由β-转角和无规卷曲构成。抑菌试验结果显示：抗菌肽对所选4株革兰氏阳性菌的MIC为7.81μg/mL,对4株革兰氏阴性菌的MIC为7.81-62.5μg/mL,对青霉、黑曲霉的MIC分别为31.25、62.5μg/mL。溶血试验结果显示：抗菌肽质量浓度≤62.5μg/mL时,其溶血率不超过10%;但抗菌肽质量浓度≥125μg/mL时,其溶血率超过80%。稳定性实验结果显示：该抗菌肽具有很强的热稳定性和p H稳定性,对蛋白酶有很好的耐受性。In order to study the structure and function of antimicrobial peptide from Brevibacillus laterosporus S62-9,the molecular mass,amino acid sequence and secondary structure of antimicrobial peptide were analyzed by MALDI-TOF/TOF mass spectrometry and FT-IR. The results indicated that the molecular mass of the peptide is 1 602.004 4 Da, and the primary structure of the peptide contains eleven amino acid residues,L-Y-K-L-V-K-V-V-L-N-M with a choline and a tenuazonic acid connected to the N-terminal and the C-terminal, respectively. The secondary structure mainly consists of β -turn and random coil. Antimicrobial assay indicated that the peptide is able to inhibit the growth both Gram-positive and Gram-negative bacteria with the MIC values of 7.81 μg/mL and 7.81-452.5 μg/mL respectively. The peptide also can inhibit the growth of the Penicillium sp and Aspergillus niger with the MIC value of 31.25 μg/mL and 62.5 μg/mL, repectively. The hemolytic results showed that the peptide exhibited hemolytic activity below 10% at 62.5 μg/mL,and more than 80% at 125μg/mL. In addition ,the peptide showed high thermal and pH stability and a strong resisted proteinases.

关 键 词：侧孢短芽孢杆菌 抗菌肽 结构 性质 

分 类 号：Q936[生物学—微生物学]