携带IL-7的新城疫病毒修饰的自体肿瘤疫苗对肝癌的免疫治疗效应  

The immunotherapeutic effects of autologous tumor vaccine modified with recombinant Newcastle disease virus expressing IL-7 in hepatoma

作　　者：郭灵华[1] 赵李祥[1] 梅雨[1] 胡博[1] 刘海燕[1] GUO Linghua ZHAO Lixiang MEI Yu HU Bo LIU Haiyan(Laboratory of CeUular and Molecular Tumor Immunology, Soochow University, Suzhou 215123, China)

机构地区：[1]苏州大学医学部细胞与分子肿瘤免疫实验室,215123

出　　处：《免疫学杂志》2016年第12期1034-1038,共5页Immunological Journal

摘　　要：目的研究携带IL-7的新城疫病毒修饰的自体肿瘤疫苗的抗肝癌作用。方法鸡胚扩增新城疫病毒LX/(IL-7),体外感染Hepa1-6细胞,显微镜下观察LX/(IL-7)的裂解性特征,并采用ELISA的方法检测感染Hepa1-6细胞后IL-7的表达水平;将C57BL/6小鼠分为2组,每组5只,通过皮下接种5×106个Hepa1-6细胞建立小鼠肝癌模型。成瘤1周后,200μg/ml丝裂霉素C处理的2×107/ml Hepa1-6细胞感染LX/(IL-7)病毒制成肿瘤疫苗。对照组小鼠皮下接种丝裂霉素C处理的Hepa1-6细胞,每只接种1×106个细胞,实验组接种瘤苗,每只接种1×106个细胞,每周2次。测量肿瘤体积,通过流式检测小鼠脾脏细胞免疫反应。结果 LX/(IL-7)病毒具有非裂解性特征,可以高效地感染肝癌细胞并分泌IL-7。肝癌皮下模型的结果表明LX/(IL-7)修饰的Hepa1-6瘤苗具有较好的肿瘤免疫治疗效果,流式结果显示其可增强效应T细胞的比例,促进T细胞的免疫应答。结论携带IL-7的新城疫病毒修饰的自体肿瘤疫苗具有显著抗肝癌作用。This study was designed to clarify the anti-hepatoma effects of autologous tumor vaccine modifiedwith recombinant Newcastle disease virus expressing IL-7. The Newcastle disease viruses LX/（IL-7） were amplifiedin chick embryo, and then their schizolysis were observed by microscope through hepatoma carcinoma cell infectionin vitro. To determine whether the IL-7 gene was inserted into LX, the Hepa1-6 cells infected with LX/（IL-7） weredetected for IL-7 secretion by ELISA. C57B/L6 mice were used to establish hepatoma model by subcutaneouslyinoculation of 5×106Hepa1-6 cells. One week later, 200 μg/ml mitomycin C-treated Hepa1-6 cells infected withLX/（IL-7） as modified tumor vaccine cells were inoculated subcutaneously into tumor-bearing mice twice a week.The mice immunized with the vaccine modified by LX/（IL-7） have smaller tumor size than that immunized withcontrol vaccine cells. Vaccination with the vaccine modified by LX/（IL-7） could significantly enhance effective Tcells percentage, and promote T cells immune response. Overall, autologous tumor vaccine modified by Newcastledisease viruse carried IL-7 has significant anti-hepatoma function.

关 键 词：新城疫病毒 ATV-NDV IL-7 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]