pH及溶氧对重组大肠杆菌发酵生产5-氨基乙酰丙酸的影响  

Effect of p H and dissolved oxygen on the fermentation production of 5-aminolevulinic acid by recombinant Escherichia coli

作　　者：程雪莲[1] 王倩[1] 祁庆生[1,2] CHENG Xuelian WANG Qian QI Qingsheng(National Glycoengineedng Center, Shandong University, Jinan 250100, China State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China)

机构地区：[1]山东大学国家糖工程技术研究中心,山东济南250100 [2]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《中国酿造》2016年第11期145-148,共4页China Brewing

基　　金：国家自然科学基金(31370085);国家863计划项目子课题(2012AA022104);山东省自主创新及成果转化专项(201422CX02602)

摘　　要：以重组大肠杆菌DALA为实验菌株，研究了该菌株在机械搅拌通风发酵罐发酵过程中pH以及溶解氧对5-氨基乙酰丙酸（ALA）积累的影响。结果发现，发酵前期（0-27h）pH保持为6．51稳定期后期（28-48h），pH为6．0时有利于5．ALA的积累。其次，通过控制转速与通气量调节发酵液中的溶氧，发现发酵前期转速为500r／min，通气量为2vvm；稳定期后期，转速降低至250r／min，通气量减少为1vvm，有利于重组菌DALA发酵生产5．ALA，在此条件下发酵5-ALA的产量可达到3．46g／L。Using the recombinant Escherichia coli DALA as experimental strains, the effects ofpH and dissolved oxygen on the 5-aminolevulinic acid （5-ALA） during fermentation in mechanical stirred fermentation tank were researched. The results showed that the pH was 6.5 in the early period of fermentation （0-27 h）, and in the late period of stable stage （28-48 h）, the pH was 6.5, which was conducive to the accumulation of 5-ALA. Besides, by controlling the rotate speed and the aeration to adjust dissolved oxygen in the fermentation liquid, it showed that in the early period of fermentation, the rotate speed was 500 r/rain and aeration was 2 vvm. In the late period of stable stage, the rotate speed and aeration were reduced to 250 r/min and 1 vvm, respectively, which was conducive to the fermentation production of 5-ALA by recombinant E. coli. Under the conditions, the yield of 5-ALA was up to 3.46 g/L.

关 键 词：重组大肠杆菌 5-氨基乙酰丙酸 溶解氧 

分 类 号：Q939.99[生物学—微生物学]