河北地区蚯蚓种群遗传多样性分析  

Analysis of Genetic Diversity of Drawida gisti and Soil Properties

作　　者：张玉峰[1] 刘莹莹[1] 李丛胜[1] 毕艳孟[2] 申磊[1] ZHANG Yu-feng LIU Ying-ying LI Cong-sheng BI Yan-meng SHEN Lei(College of life Sciences, Langfang Teachers University, Langfang 065000, China College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China)

机构地区：[1]廊坊师范学院生命科学学院,河北廊坊065000 [2]中国农业大学资源与环境学院,北京100193

出　　处：《河北农业科学》2016年第4期45-48,共4页Journal of Hebei Agricultural Sciences

基　　金：河北省自然科学基金项目(C2015408038);河北省大学生创新创业训练项目(201510100034);河北省高等学校科学技术研究项目(QN2014055);廊坊师范学院博士自然科学基金项目(LSBS201305)

摘　　要：为了探讨蚯蚓遗传多样性与土壤环境的关系,2015年6-10月在河北地区进行蚯蚓资源调查和样品采集,利用PCR技术扩增线粒体基因16S r DNA、ND1序列,分析了该区蚯蚓优势种类天锡杜拉蚓（Drawida gisti）的遗传多样性。结果表明：来自河北23个采集点的研究样本,16S、ND1全部DNA序列的平均单倍型多样性分别为0.76和0.64,平均核苷酸多样性分别为0.042和0.039,群体间平均遗传距离分别为0.045和0.054。土壤性质显著影响种群的遗传变异水平。The study aimed to discussing relation between earthworm genetic diversity and soil environ-ment. Based on field studies conducted from July to October 2015,it was analyzed the genetic diversity of Drawida gisti using mitochondrial 16 S and ND1 genes which were amplified by PCR in samples ob-tained from twenty-three localities,DNA polymorphism within and between groups was analyzed. The average haplotye diversity based on 16 S and ND1 genes were 0.76 and 0.64,respectively,mean nucleotide diversity were 0.042 and 0.039, respectively, mean genetic distance between groups were 0.045 and0.054,respectively. Genetic variation was significantly influenced by soil properties.

关 键 词：蚯蚓 天锡杜拉蚓 遗传多样性 土壤性质 河北地区 

分 类 号：Q958[生物学—动物学]