miR-19通过靶定PDE4A促进肝癌细胞增殖  

Effect of High Expressed miR-19 on the Proliferation of Liver Cancer Cells Through the Target Gene of PDE4A

作　　者：袁丽杰[1] 曲艺林[1] 刘兴汉[1] 赵恒宇[2] 

机构地区：[1]哈尔滨医科大学大庆校区 [2]大庆油田总医院,黑龙江大庆163319

出　　处：《黑龙江医药》2016年第6期1041-1044,共4页Heilongjiang Medicine journal

基　　金：黑龙江省教育厅课题(NO 11541154)

摘　　要：目的:探讨miR-19对肝癌细胞系MHCC97H和LM3增殖的影响和肿癌组织中的表达情况,以及确定其靶基因和功能。方法:Real-time PCR检测miR-19的表达。WST-1和PI染色分析MHCC97H和LM3细胞的增殖水平。生物信息学预测、Luciferase以及Real-time PCR和western blot验证miR-19下游靶基因。结果:miR-19在肝癌组织中表达下调(P<0.01)。miR-19可以抑制肝癌细胞系MHCC97H和LM3增殖能力。PDE4A作为miR-19的候选靶基因,其通过诱导PDE4A上调促进肝癌细胞增殖。结论:miR-19的直接靶基因是PDE4A,下调miR-19促进PDE4A的表达,进而促进肝癌细胞的增殖。Objective： To investigate the expression level of miR-19 in HCC tissues; the role of miR-19 on the liver cancer cell proliferation and identify the target gene for miR-19 in colon cancer cells. Methods： The expression of miR-19 were detected by real time PCR. The proliferation ability and migration ability of the transfected liver cancer cells were measured by WST-1 and PI staining assay methods.The bioinforamtics was used to predict the target gene for miR-19, and Luciferase-EGFP reporter assay, real time PCR and western blot were performed to validate the target gene. Results： miR-19 was down-regulated in the liver carcinoma tissues, compared to the adjacent nontumor tissues （P〈0.01）. PDE4A was predicted as a candidate target gene for miR-19, miR-19 down-regulated promote PDE4A expression on the mRNA and protein levels （P〈0.01）, in contrast, overexpression of miR-19 had the opposite effect. Finally, the overexpression of PDE4A promots the liver cancer cells viability ability （P〈0.01）. Conclusion：PDE4A is a direct target gene for miR-19, and miR-19 promots the cell proliferation by upregulation of PDE4A.

关 键 词：微小RNA 肝癌 增殖 PDE4A 

分 类 号：R735.7[医药卫生—肿瘤]