切取活检对裸鼠舌部Tca8113移植瘤生物学行为影响的研究  

Exploratory study on influence of biopsy to biological behavior of Tca8113 transplanted tumor

作　　者：刘济远[1] 潘剑[1] 华成舸[1] 吴云龙[1] 罗正文[1] 唐休发[2] 

机构地区：[1]口腔疾病研究国家重点实验室,华西口腔医院牙槽外科(四川大学),成都610041 [2]口腔疾病研究国家重点实验室,华西口腔医院头颈肿瘤外科(四川大学),成都610041

出　　处：《华西口腔医学杂志》2016年第6期626-631,共6页West China Journal of Stomatology

基　　金：四川省科技厅项目(2014SZ0204)~~

摘　　要：目的通过建立裸鼠Tca8113移植瘤切取活检动物模型来了解活检对肿瘤生物学行为的影响,并初步探讨其机制。方法将裸鼠分为两组。舌部注射Tca8113细胞,成瘤后建立切取活检模型。观测裸鼠生存情况,原发灶生长及淋巴结转移状况,并通过免疫组织化学染色检测肿瘤原发灶核因子κB(NF-κB)、基质金属蛋白酶-9(MMP-9)、血管内皮生长因子(VEGF)、基质衍生因子-1(SDF-1)、细胞增殖抗原标记物(Ki67)表达水平及肿瘤淋巴结微转移情况。结果移植瘤成瘤率达97.92%。切取活检后实验组原发灶生长速度,淋巴结转移率,微血管密度,NF-κB、MMP-9及SDF-1表达均高于对照组。各项指标表达具有一定相关性。结论切取活检对肿瘤的生物学行为具有一定影响,可能加速原发灶的生长及转移。Objective We established an animal model of nude mice with Tca8113 tumor and cut some tissue for biopsy. We also determined the biological behavior and mechanisms of the tumor. Methods The mice were divided into two groups randomly. Mice in both groups were injected with Tca8113 cells into their tongues. The survival condition, growth of primary focus, and metastasis were observed. Hematoxylin and eosin staining and immunohistochemistry were performed on nuclear factor κB （NF-κB）, matrix metallopeptidase 9 （MMP-9）, vascular endothelial growth factor （VEGF）, stromal cell-derived factor 1 （SDF-1）, and Ki67 to determine their distributions within the tumor. Cytokeratin staining was also performed to detect micrometastasis in the submandibular lymph nodes. Results The emerging rate of tumor was 97.92%. The weight and survival time of the experimental group were lower than that of the control group, whereas the metastasis ratio was higher. The expression ofNF-κB, MMP-9, SDF-1, and MMP-9 in tumors was higher in the experimental group than that in the control group. The expression of NF-κB, MMP-9, VEGF, and SDF-1 was relevant. The microvessel density of the experimental group was higher than that in the control group. Conclusion Biopsy can affect the biological behavior of tongue tumor and can promote growth of primary focus and metastasis.

关 键 词：舌癌 活检 肿瘤生长 肿瘤转移 

分 类 号：R739.8[医药卫生—肿瘤]