糖尿病大鼠的视网膜和脉络膜中VEGFR-2的表达  

Expression of VEGFR-2 in the Retina and Choroid of Diabetic Rats

作　　者：罗文婷[1] 张晓元[2] 张中宇[3] 张雪[3] 闫朝岐[4] 谢芳[5] 付红梅[1] 李丹丹[1] 赵兴鹃[1] 孙大卫[3] 

机构地区：[1]哈尔滨医科大学第二附属医院体检中心,黑龙江哈尔滨150086 [2]哈尔滨医科大学第二附属医院心内科,黑龙江哈尔滨150086 [3]哈尔滨医科大学第二附属医院眼科,黑龙江哈尔滨150086 [4]哈尔滨医科大学第二附属医院乳腺外科,黑龙江哈尔滨150086 [5]哈尔滨医科大学第一附属医院眼科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2016年第34期6610-6613,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81171381)

摘　　要：目的:研究早期糖尿病大鼠视网膜和脉络膜血管中VEGFR-2的表达。方法:24只雄性Wistar大鼠,随机分为两组:正常对照组和糖尿病组。实验组给予一次性静脉注射60 mg/kg STZ建立糖尿病模型。STZ注射后24 h,血糖水平超过250 mg/dL被认为是糖尿病模型。WB技术检测VEGFR-2在视网膜和脉络膜的表达水平,免疫组化法用来定位VEGFR-2。结果:STZ注射14天后,糖尿病组的平均体重较对照组显著降低(糖尿病组:177±10 g,对照组:243±19 g,P<0.05),糖尿病组的平均血糖水平较对照组显著增高(糖尿病组的血糖为498±36 mg/m L,对照组的血糖为90±10 mg/m L,P<0.05)。VEGFR-2在糖尿病鼠的视网膜和脉络膜中表达较对照组增加,其差异有统计学意义(P<0.05)。免疫组化分析显示:VEGFR-2在糖尿病组的视网膜和脉络膜血管中表达显著增加,而在正常对照组中少量表达(P<0.05)。结论:VEGFR-2在试验诱导的糖尿病模型的视网膜和脉络膜血管中的表达增加,VEGFR-2可能成为糖尿病视网膜病变新的诊断靶点。Objective： To research the expression of VEGFR-2 in the retinal and choroidal vessels of diabetic rats in early stage. Methods： 24 male Wistar rats were divided into two groups randomly： normal control group and diabetic group. The experimental group was given a one-time intravenous injection of STZ （60 mg/kg） to establish diabetic model. Animals with blood glucose levels higher than 250 mg/dL 24 h after STZ injection were considered diabetic model. Western Blot was used to detect the expression of VEGFR-2 in the retina and choroid. Immunohistochemical staining method was used to localize VEGFR2. Results： 14 days after STZ-injection average body weight of diabetic rats showed significantly lower compared with the normal controls （diabetic rats： 177±10 g, normal controls： 243± 19 g, P〈0.05 ）; average blood glucose of diabetic rats showed significantly higher compared with the normal controls （diabetic rats： 498±36 mg/mL, normal controls： 90± 10 mg/mL,P〈0.05 ）. Compared with the control group, the expression of VEGFR-2 in the retinal and choroidal vessels of diabetic rats was increased, and the difference was statistically significant （P〈0.05）. The immunohistochemical analysis showed significantly increased VEGFR-2 expression in the retinal and choroidal vessels of the diabetic group, whereas the VEGFR-2 of the retina and choroid in the control group was little （P〈0.05）. Conclusions： The findings suggest that VEGFR-2 expression is increased in retinal and choroidal vessels in experimentally induced diabetes. VEGFR-2 may be a target for the diagnosis of diabetic retinopathy.

关 键 词：糖尿病视网膜病变 VEGFR-2 STZ 血管内皮生长因子 

分 类 号：R-33[医药卫生]