甜樱桃‘佐藤锦’果实生长发育过程AsA含量及其相关酶活性的变化  

Changes of AsA Content and Related Enzyme Activities in Sweet Cherry‘Satonishiki'Fruit during Development

作　　者：夏惠[1] 林玲[2] 高帆[2] 倪知游 高丽扬[2] 吕秀兰[1] 梁东[1] 

机构地区：[1]四川农业大学果蔬研究所,成都611130 [2]四川农业大学园艺学院,成都611130

出　　处：《西北植物学报》2016年第10期2008-2014,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：四川省教育厅重点项目(16ZA0024);四川农业大学学科建设双支计划(2015年)

摘　　要：以黄肉甜樱桃品种‘佐藤锦’为材料,测定了其果实生长发育过程中抗坏血酸(AsA)和谷胱甘肽(GSH)的含量变化,及其相关代谢酶L-半乳糖脱氢酶(GalDH)、L-半乳糖-1-4-内酯酶(GalLDH)、单脱氢抗坏血酸还原酶(MDHAR)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)和过氧化物酶(APX)的活性变化,分析它们在果实生长发育过程中对AsA积累所起的作用。结果表明:(1)‘佐藤锦’果实生长发育过程中总抗坏血酸(T-AsA)、还原型抗坏血酸(AsA)、脱氢抗坏血酸(DHA)、氧化型谷胱甘肽(GSSG)含量均在花后0d最高,随后持续下降,而总谷胱甘肽(T-GSH)和还原型谷胱甘肽(GSH)含量先升后降。(2)随着果实生长发育,AsA和DHA的单果积累量均持续增加,且在果实第二次快速生长期增幅最大;各相关代谢酶活性在甜樱桃果实生长发育过程中呈现出不同的变化趋势,其中GalLDH、MDHAR和DHAR的活性变化同AsA含量变化趋势基本一致。(3)相关性分析发现,GalLDH、MDHAR和DHAR的活性与AsA含量呈极显著正相关关系,说明它们是影响甜樱桃果实AsA含量的关键酶。In this paper, the contents of ascorbic acid （AsA）, glutathione （GSH）, and related enzyme ac- tivities of L-galactose dehydrogenase （GalDH）, L-galactono-1-4-lactone dehydrogenase （GalLDH）, mono- dehydroascorbate reductase （MDHAR）, dehydroascorbate reductase （DHAR）, glutathione reductase （GR） and ascorbic acid peroxidase （APX） were investigated during the fruit development in sweet cherry‘Satonishiki＇. The results showed that： （1） the contents of total AsA, AsA, DHA and GSSG was the highest at the 0 day after anthesis （DAA）, and then decreased persistently. However, the contents of to- tal GSH and GSH was first increased and then decreased. （2） The accumulation of AsA and GSH increased during the whole growth process of sweet cherry fruit, especially during the second rapid growth period of fruit development. Different enzymes involved in metabolism of AsA showed different patternsduring the development of sweet cherry fruit, but the changes of GalLDH, MDHAR and DHAR actlvmes were similar to that of AsA. （3） The correlation analysis showed that they had a significantly positive cor- relation with the content of AsA, which indicated that they may he the key enzymes of AsA accumulation in sweet cherry fruit.

关 键 词：甜樱桃 果实 抗坏血酸 代谢酶活性 

分 类 号：Q945.64[生物学—植物学]