单胺类及5-羟色胺拮抗剂对海湾扇贝幼虫生长的影响  

Effects of Monoamines and 5-HT Antagonists on Larval Growth in Bay Scallop

作　　者：马斌[1] 刘全圣 王伟[1] 赵侠[1] 赵玉明[1] 王春德[1] 

机构地区：[1]青岛农业大学海洋科学与工程学院,山东青岛266109

出　　处：《水产科学》2016年第6期620-624,共5页Fisheries Science

基　　金：国家自然科学基金面上项目(31572168);山东省农业重大应用技术创新项目;山东省现代农业产业技术体系贝类创新团队建设项目

摘　　要：单胺类药物左旋多巴胺和5-羟色胺一方面可能促进海湾扇贝幼虫纤毛活动从而促进幼虫摄食,另一方面引起纤毛运动强度的改变,又影响幼虫的能量消耗,因此对扇贝幼虫的生长可能存在着双重影响。以海湾扇贝幼虫为研究对象,探讨了左旋多巴胺和5-羟色胺及5-羟色胺的两种拮抗剂曲唑酮和赛庚啶对其生长的影响及其可能的作用机制。试验结果表明,0.1ng/L左旋多巴胺和1.0ng/L 5-羟色胺对海湾扇贝幼体的生长率促进作用最强,而5-羟色胺的促生长作用可被曲唑酮和赛庚啶抑制,说明5-羟色胺可能是通过5-HT1或5-HT2型受体起作用的。在海湾扇贝的育苗实践中可以选择使用低质量浓度的5-羟色胺以促进幼虫的生长。L-dopamine and serotonin （5-HT） are routine monoamine neurotransmitters that may have dual effects on the larval growth of the bay scallop Argopecten irradians irradians. They may accelerate larval growth by stimulating cilia beating and thus feeding while inhibition of larval growth may occur as a result of extra energy cost caused by accelerated ciliary movements. This study aimed to determine the ultimate outcome of the dual effects of L-dopamine and 5-HT on larval growth and also explore the underlying mechanism by examining the actions of 5-HT and its antagonists, trazodone and cyproheptadine. The results showed that larval growth was most accelerated by 0.1 ng/L L-dopamine and 1.0 ng/L 5-HT. The blockade of the 5-HT actions by its antagonists suggests that 5-HT may exert its effects via its binding to 5-HT2A receptor. These monoamines may be potentially used to accelerate larval growth in scallop culture.

关 键 词：海湾扇贝 幼虫生长 左旋多巴胺 5-羟色胺 曲唑酮 赛庚啶 

分 类 号：S968.313[农业科学—水产养殖]