阿司匹林可抑制吗啡引起的HT22细胞Wnt通路的激活  

Morphine Induce the Activity of Wnt Signaling in HT22 Cells Which can be Inhibited by Aspirin

作　　者：王建材 张兴业[1] 祝刚[1] 李泉 高国栋[1] 罗涛[1] 

机构地区：[1]第四军医大学唐都医院神经外科,陕西西安710038 [2]西安市胸科医院重症医学科,陕西西安710100

出　　处：《现代生物医学进展》2016年第33期6410-6413,共4页Progress in Modern Biomedicine

基　　金：国家重点基础研究发展计划项目(973计划)(2011CB51000);唐都医院科技创新发展基金重大国际合作项目(2013GJHZ001)

摘　　要：目的:研究阿司匹林对吗啡引起的Wnt通路的上调的影响。方法:在大鼠海马神经元元细胞系HT22细胞上建立了慢性吗啡成瘾模型,然后运用免疫荧光技术和Western blot技术检测wnt通路中β-catenin蛋白的表达变化变化,同时应用报告基因技术检测Wnt通路下游转录因子的活性。结果:在慢性吗啡成瘾模型组,β-catenin蛋白的表达显著增加,而在接受了6 h的阿司匹林预处理的慢性吗啡成瘾模型组,β-catenin蛋白的表达受到了显著的抑制。结论:阿司匹林可抑制吗啡引起的Wnt/β-catenin通路水平的过表达。Objective： To analyse the role of aspirin in down-regulation the Wnt signaling which activated by morphine addiction.Many studies have revealed that Wnt signaling activated in morphine addiction rats models. Methods： Many technologies such as Immunofluorescence, western blot were carried out. We also used the Luciferase test to study the downstream gene change in Wnt signaling after morphine addiction with or without aspirin pretreatment. Results： We found that the level of β-catenin increased in chronic morphine addiction group. However, the use of 6h aspirin before adding morphine leads to the down-regulation of β-catenin. Conclusions：Our study identified that aspirin can inhibited the Wnt signaling which activated after chronic morphine addiction and this may be important in the cure of morphine addiction.

关 键 词：HT22细胞 WNT通路 Β-CATENIN 成瘾 吗啡 

分 类 号：R-33[医药卫生] R96