电针对失神经骨骼肌萎缩大鼠胰岛素样生长因子1、肌肉生长抑制素及肌卫星细胞增殖的影响  

Effects of Electroacupuncture on Insulin-like Growth Factor-1, Myostatin and Satellite-cell Proliferation in Rats with Denervated Skeletal Muscle Atrophy

作　　者：高睿琦 唐成林[1] 曹净[1] 郭全虎[1] 张毅[1] 田源[1] 袁海洲[1] 

机构地区：[1]重庆医科大学中医药学院,重庆市400016

出　　处：《中国康复理论与实践》2016年第11期1259-1263,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目(No.81273870);重庆市卫计委中医药科研项目(No.ZY20150249)

摘　　要：目的探讨电针延缓失神经骨骼肌萎缩的可能机制。方法 49只雄性Sprague-Dawley大鼠随机分为正常组(A组,n=7)、自然恢复组(B组,n=21)和电针治疗组(C组,n=21)。A组不做处理,其余两组切断坐骨神经制备失神经性骨骼肌萎缩模型。术后1 d,C组术侧腓肠肌给予电针足三里、承山治疗每天1次。术后7 d、14 d、21 d分别测定术侧腓肠肌湿重比,HE染色测定肌纤维直径及截面积,Western blotting检测胰岛素样生长因子1(IGF-1)、肌肉生长抑制素(Myostatin)以及增殖细胞核抗原(PCNA)蛋白,RT-PCR检测IGF-1、Myostatin和PCNA基因表达。结果与A组比较,B组和C组腓肠肌湿重比、肌纤维截面积及直径均显著下降(P<0.001),但C组显著高于B组(P<0.001)。C组IGF-1、PCNA蛋白和基因表达高于B组(P<0.05),Myostatin蛋白和基因表达低于C组(P<0.05)。结论电针能有效促进IGF-1的表达,抑制Myostatin的表达,从而促进肌卫星细胞增殖。这可能是电针延缓失神经性骨骼肌肌萎缩的机制之一。Objective To observe effects and mechanism of electroacupuncture（EA） on denervated skeletal muscle atrophy. Methods Forty-nine male Sprague-Dawley rats were randomly divided into normal group（group A, n=7）, natural recovery group（group B, n=21） and EA group（group C, n=21）. The groups B and C, established the model of denervated skeletal muscle atrophy by transecting the sciatic nerve of rats, were divided into subgroups of 7 days, 14 days, 21 days postoperation, seven in each subgroup. Electroacupuncture was given to the group C at Zusanli（ST36） and Chengshan（BL57） once a day since 24 hours after modeling. The muscle wet weight ratio of the affected gastrocnemius was determined. Cross-sectional area and fiber diameter of the gastrocnemius were measured with HE staining. The expression of insulin-like growth factor-1（IGF-1）, Myostatin and proliferating cell nuclear antigen（PCNA） protein and gene in the gastrocnemius were detected with Western blotting and RT-PCR. Results The wet weight ratio, cross-sectional area and fiber diameter were less in the groups B and C than in the group A（P〈0.001）, and they were more in the group C than in the group B（P〈0.001）. Compared with the group B, the protein and gene of IGF-1, PCNA increased in the group C（P〈0.05）, while the Myostatin decreased（P〈0.05）. Conclusion Electroacupuncture can increase the expression of IGF-1 and decrease the expression of Myostatin, to promote the proliferation of satellite cell, which may relate with the prevention of denervated skeletal muscle atrophy.

关 键 词：失神经 骨骼肌萎缩 电针 胰岛素样生长因子1 肌肉生长抑制素 增殖细胞核抗原 肌卫星细胞 大鼠 

分 类 号：R746.4[医药卫生—神经病学与精神病学]