Bcl-2及Bax在大鼠腺体萎缩过程中的表达及临床意义  

The expression of Bcl-2 and Bax proteins and their significance with the atrophy of parotid gland cells in rats

作　　者：高红[1] 左金华[1] 王丽芳[2] 朱玉红[2] 杨勇[2] 王芳[2] 宋冰[2] 毛玉龙[2] 

机构地区：[1]滨州医学院附属医院口腔颌面外科,256603 [2]滨州医学院附属医院病理科,256603

出　　处：《实用口腔医学杂志》2016年第6期758-762,共5页Journal of Practical Stomatology

摘　　要：目的：探讨大鼠腮腺主导管结扎后B cell lymphoma 2（Bcl-2）、Bcl-2-associated X protein（Bax）在腺体萎缩过程中的表达及临床意义。方法：对72只Wistar大鼠进行不同时间点腮腺主导管结扎建立腮腺萎缩模型;分别于术后1、3、5、7、14、21、30、60、90、150、180 d取材,采用免疫组织化学染色法检测Bcl-2、Bax在不同时间点的表达及分布情况。结果：主导管结扎后7 d多数腺泡细胞萎缩消失。Bcl-2和Bax在正常腺体组织中呈低水平表达。主导管结扎后不同时间点Bcl-2和bax在腺体中呈高表达,3 d时Bax表达达到峰值（1.99±0.10）,21 d时Bcl-2的表达达到峰值（3.02±0.10）,随后Bcl-2及Bax表达均降低。Bcl-2/Bax比值在1~21 d升高,随后降低并趋于稳定。结论：腮腺主导管结扎后Bax、Bcl-2的表达与腮腺萎缩过程相关。Objective： To investigate the expression and correlation of Bcl-2 and Bax in the parotid gland after leading duct ligation in rats. Methods： Atrophy of the right parotid was induced by ligating the right main duct of 72 rats. Immunohistochemical labelling was performed to study the expression of Bcl-2 and Bax 1,3,5, 7, 14, 21,30, 60, 90, 150 and 180 clays after duct ligation. Results： 7 d after duct ligation most acinar cells disappeared. The distribution of Bcl-2 and Bax protein in normal parotid was in cytoplasm with unifo- maity. Bcl-2 and bax higher expression was identified in the gland at all time points. The expression of Bcl-2 protein was significantly in- creased and reached the peak at 21 d after duct ligation. More Bax-positive acinar cells on day 3 were observed, then the expression of Bcl-2 and Bax protein was descended. Higher Bcl-2/Bax ratio was identified at 1 -21 d, then descended. Conclusion： The expression of Bcl-2 and Bax is associated with the atophy of the parotid glad after rat parotid duct ligation.

关 键 词：腮腺 细胞凋亡 BCL-2基因 BAX基因 

分 类 号：R781.7[医药卫生—口腔医学]