IGF-1对体外培养髁突软骨细胞增殖与凋亡的影响  

The effects of IGF-1 on the proliferation and apoptosis of cultured condylar chondrocytes

作　　者：罗锐[1] 张瑞智[1] 龚正林[1] 张萍[1] 

机构地区：[1]安康市中心医院口腔颌面外科,725000

出　　处：《实用口腔医学杂志》2016年第6期810-814,共5页Journal of Practical Stomatology

摘　　要：目的：研究IGF-1对IL-1β诱导的颞下颌关节软骨细胞增殖与凋亡的影响。方法：组织块培养法分离培养人髁突软骨细胞,通过细胞形态观察和免疫细胞化学染色进行鉴定。将培养的细胞分为：对照组、IL-1β组（10μg/L）、IL-1β＋IGF-1组（0、1、10、50、100μg/L）,MTT法检测各组细胞增殖能力变化,流式细胞仪检测细胞周期分布以及细胞凋亡情况,Western blot检测各组细胞中凋亡相关因子Bcl-2、Bax和Caspase-3以及p38 MAPK/NF-κB蛋白表达变化。结果：人髁突软骨细胞生长状态良好,甲苯胺蓝染色胞质呈深蓝色,II型胶原呈阳性表达。与对照组比较,IL-1β组细胞增殖能力、Bcl-2/Bax比值明显降低,早凋与晚凋细胞百分数、Caspase-3、p38 MAPK/NF-κB蛋白表达均明显增加;与IL-1β组比较,1~100μg/L IGF-1预处理组细胞增殖能力、Bcl-2/Bax比值逐渐上升（P〈0.05）,细胞凋亡率、Caspase-3、p38 MAPK/NF-κB蛋白表达则逐渐下调（P〈0.05）,均呈现一定的浓度依赖性。结论：IGF-1可抑制IL-1β诱导的髁突软骨细胞凋亡并减轻p38 MAPK/NF-κB的活化。Objective： To investigate the effects of IGF-1 and IL-1β on the proliferation and apoptosis of cuhured human condylar chondrocytes （CCs） of temporomaudibular j oint（TMJ）. Methods： Cultured CCs were derived from human TMJ condylar cartilage tis- sue, and identified by immunocytochemistry staining. The cultured cells were divided into 6 groups： control group, IL-1β（ 10μg/L） group and IL-1 group （10 μg/L） ＋ IGF-1 group （0, 1, 10, 50 and 100 μg/L, respectively）. The cell proliferation ability was de- tected by MTT assay. The cell cycle and apoptosis were detected by flow cytometry. The expression of apoptosis-associated factors Bcl- 2, Bax and p38 MAPK/NF-KB proteins were detected by Western blot. Results： Type II collagen was positively expressed in cultured CCs. IL- 1β treatment decreased cell proliferation, increased cell apoptosis with concomitant increase of the percentage of early apopto- sis and late apoptotic cells, increased Caspase-3 expression, decreased Bcl-2/Bax ratio, and increased the expression of p38 MAPK/ NF-KB proteins. Whereas, with 1 -100μg/L IGF-1 pretreatment, the proliferation ability and Bcl-2/Bax ratio of the cells were in- creased（P 〈 0.05）, the apoptotic cells were decreased, the expression of Caspase-3 and p38 MAPK/NF-κB proteins was decreased （ P 〈 0.05 ） in a dose- dependent manner. Conclusion： IGF-1 may inhibit IL- 1 β induced cell apoptosis and attenuate the activation of p38 MAPK/NF-KB of human condylar chondrocytes.

关 键 词：IGF-1 IL-1Β 增殖 凋亡 P38 MAPK/NF-κB 

分 类 号：R782.6[医药卫生—口腔医学]