IFN-γ+874A/T位点和IL-2-330T/G位点的单核苷酸多态性与复发性口腔溃疡易感性的关系  

Relationship between IFN-γ + 874A/T site and IL-2-330T/G site single nucleotide polymorphism with the susceptibility of recurrent aphthous ulcer

作　　者：张敬[1] 沙晶晶[1] 龚娟[1] 

机构地区：[1]宁夏医科大学总医院,银川750004

出　　处：《实用口腔医学杂志》2016年第6期852-856,共5页Journal of Practical Stomatology

基　　金：宁夏自然基金项目(编号:NZ201322);宁夏高等学校科学研究项目(编号:NGY2014072);宁夏医科大学校级科研项目(编号:XZ2016014)

摘　　要：目的:研究IFN-γ+874A/T位点与IL-2-330T/G位点的单核苷酸多态性(SNP)与复发性口腔溃疡(RAU)易感性的关系。方法:采用ELISA方法检测138例RAU组患者和124例正常对照组中IFN-γ及IL-2的血清含量,并用SSP-PCR和RFLPPCR对全血进行IFN-γ+874位点与IL-2-330位点的单核苷酸多态性的检测分析。结果:RAU组患者血清中IFN-γ及IL-2水平均显著低于正常对照组(P<0.05)。IFN-γ+874位点在基因型频率与等位基因频率的分布2组间差异有显著性(P<0.05)。IFN-γ+874位点基因型AA的OR值=9.964为易感基因型,A等位基因为易感等位基因,OR值=3.801。IL-2-330位点在基因型频率与等位基因频率的分布2组间差异无显著性(P>0.05)。IFN-γ+874位点中,携带A等位基因患者患复发性口腔溃疡的风险是携带T等位基因者的3.801倍。结论:IFN-γ+874位点中携带A的等位基因与RAU易感性相关,IL-2-330位点与RAU的易感性无关。Objective： To explore the possible relationship between recurrent aphthous ulcer（RAU） and the single nucleotide polymor- phism（SNP） of IFN-γ ＋ 874A/T site and IL-2-330T/G site. Methods： 138 RAU patients and the 124 health control subjects were in- cluded. IFN-γand IL-2 serum concentration were detected by ELISA. IFN-γ ＋ 874A/T site and IL-2-330T/G site of the whole blood were detected by RFLP-PCR and SSP-PCR. Results： IFN-γ and IL-2 levels in serum of the patients were significantly lower than those of the controls （P 〈 O. 05）. There was significant difference in the genotype frequencies and allele frequencies of IFN-γ＋ 874A/T site between the 2 groups （ P 〈 0. 05）. The AA genotype （ OR = 9. 964） and the A allele （ OR = 3. 801 ） at the IFN-γ ＋ 874A/T site was with high-risk of RAU. Carry A allele in patients with risk of RAU was to carry the T allele of 3. 801 times. There was no significant differ- ence in the genotype frequencies or allele frequencies of 1L-2-330T/G site between the 2 groups （P 〉 0.05）. Conclusion： IFN-γ＋ 874A/T site was associated with the risk of RAU. But, IL-2-330T/G site was not.

关 键 词：IFN-Γ IL-2 单核苷酸多态性(SNP) 复发性口腔溃疡(RAU) 

分 类 号：R781.5[医药卫生—口腔医学]