贵州马铃薯推广品种SSR分子标记及遗传多样性分析  

Genetic Diversity Analysis of Guizhou Potato Production Varieties by SSR Molecular Marker

作　　者：颜谦[1] 滕安平 李恩宏 杨建[1] 施文娟 黄先群[1] 

机构地区：[1]贵州省马铃薯研究所/贵州省农业生物技术重点实验室,贵州贵阳550006 [2]贵州省种子管理站,贵州贵阳550002

出　　处：《西南农业学报》2016年第10期2294-2299,共6页Southwest China Journal of Agricultural Sciences

基　　金：贵州省级财政马铃薯发展资金项目“贵州省中药现代化专项‘葛根良种鉴选;规范化苗圃及示范体系建设'”[黔科合成字2013(5013)]

摘　　要：为了鉴定马铃薯品种的亲缘关系，采用10对SSR分子标记引物，对19份贵州马铃薯生产品种进行SSR分子标记及遗传多样性分析。结果表明：10对SSR引物中5对获得60条清晰条带，其中49条为多态性条带，多态性比率为81．67％，平均每个引物的多态性条带数为9．80条。19份马铃薯品种的遗传相似性系数为0．43～1．00。经聚类分析，在遗传相似系数为0．56处全部参试材料聚在一起，在遗传相似系数为0．63处可明显聚为3个类群。第Ⅰ类群包括11份材料，第Ⅱ类群包括7份材料，第Ⅲ类群只有1个材料。在相似性系数0．65处，57．89％的参加品种聚在一起，表明参试品种的遗传背景较为狭窄。In order to identify the genetic relationship of potato varieties, the 19 potato production varieties in Guizhou were used to analyze the genetic diversity by 10 SSR molecular marker primers. The results showed that only 5 of the 10 SSR primers were obtained clear and readable bands by the PCR amplification. The 60 DNA bands were obtained, including 49 polymorphic bands with a polymorphism rate of 81.67 %, number of polymorphic bands per primer was 9.80. The genetic similarity coefficients of the 19 potato varieties were from 0.43 to 1.00. UPGMA cluster analysis showed that all tested varieties were got together in similarity coefficient 0.56. They were divided obviously into three groups in the genetic similarity coefficient 0.63. The group Ⅰ included 11 varieties, the group Ⅱ included 7 varieties, and group Ⅲ only one variety. There were 57.89 % of the tested varieties still gathering together in the similarity coefficient 0.65. It meant that the genetic background of all tested varieties is relatively narrow.

关 键 词：马铃薯 品种 SSR标记 遗传多样性 

分 类 号：S532[农业科学—作物学]