雌激素对去卵巢大鼠抑郁样行为及杏仁核NGF表达的影响  

Effects of Estradiol on Depressive Behavior and Amygdaloid NGF Expression in Ovariectomized Rats

作　　者：苏晓云[1] 李文青[2] 冯春梅 贺继平[4] 崔建梅[5] SU Xiaoyu LI Wenqing FENG Chunmei HE Jiping CUI Jianmei(Department of Nursing Humanities Teaching and Research Section, Fenyang College of Shanxi Medical University, Fenyang 032200, China Department of Psychiatry, Rongfu Military Hospital of Jining, Jining 272000, China Department of Psychiatry, Mental Health Center of Guang＇ an in Sichuan Province, Guang＇ an 638（100, China Department of Anatomy Teaching and Research Section, Fenyang College of Shanxi Medical University, Fenyang 032200, China Department of Physical Education, Noah University of China, Taiyuan 030051, China)

机构地区：[1]山西医科大学汾阳学院护理学系,山西汾阳032200 [2]济宁市荣复军人医院精神科,山东济宁272000 [3]四川省广安市精神卫生中心精神科,四川广安638000 [4]山西医科大学汾阳学院解剖教研室,山西汾阳032200 [5]中北大学体育学院运动医学教研室,太原030051

出　　处：《中国医科大学学报》2016年第12期1119-1123,共5页Journal of China Medical University

摘　　要：目的通过观察雌二醇治疗对雌激素剥夺大鼠的抑郁样行为及杏仁核神经生长因子(NGF)含量的变化,探讨雌二醇治疗抑郁症的作用靶点及作用机制。方法健康成年雌性SD大鼠随机分为假手术组(SHAM组,n=10)、卵巢切除组(OVX组,n=10)及去卵巢雌二醇治疗组(OVX+E组,n=10)3组。雌二醇治疗8周后通过悬尾及糖水偏爱实验观察雌二醇对雌激素剥夺大鼠行为学的影响;随后用免疫组织化学方法测量和分析杏仁核NGF神经元的数量及面积。结果与SHAM组大鼠比较,OVX组大鼠糖水偏爱实验中糖水偏爱率显著下降(P<0.01)、悬尾实验中不动时间延长(P<0.01),血清雌二醇水平及杏仁核NGF表达均显著下降(P均<0.01);8周雌二醇治疗改善了去卵巢大鼠的抑郁样行为,与OVX组大鼠比较,血清雌激素水平及杏仁核NGF表达水平均显著增加(P均<0.01)。结论雌二醇治疗改善了去卵巢大鼠的抑郁样行为,可能与雌二醇增加去卵巢大鼠的血清雌激素水平及增强杏仁核NGF的表达有关。Objective To observe the changes of the depressive behavior and amygdaloid nucleus nerve growth factor （NGF） expression in estrogen-deprived female rats and explore the possible mechanism and targets of estradiol in depression treatment. Methods A total of 30 adult SD female rats were randomly assigned to 3 groups：sham operation group （SHAM, n = 10） ; ovariectomized group （OVX, n = 10）and ovariectomized rats treated with estradiol group （OVX＋E, n = 10）. The behavior changes were observed by tail suspension test （TST） and sucrose preference test （SPT） after 8-week estradiol treatment. Subsequently, immunohistochemical staining detect NGF expression in amygdaloid nucleus. Results Compared with the SHAM group rats, sucrose preference ratio significantly decreased in SPT （P 〈 0.01 ）, immobile time prolonged in TST （P 〈 0.01 ） ,serum estradiol level and amygdaloid NGF expression significantly decreased （all P 〈 0.01 ）. 8-week estradiol treatment ameliorated depression-like behavior and increased serum E2 level and NGF expression in amygdaloid nucleus in OVX＋E group rats when compared with the OVX group （all P 〈 0.01 ）. Condusion Estradiol treatment can improve the depressive behavior of ovariectomized rats, which may be related to the increase of serum estradiol level and the expression of NGF in amygdaloid nucleus.

关 键 词：雌二醇 神经生长因子 杏仁核 抑郁 

分 类 号：R749[医药卫生—神经病学与精神病学]