二氢槲皮素与二氢杨梅素的抗肿瘤活性对比  

Antitumor activity of Dihydroquercetin and Dihydromyricetin

作　　者：王佳奇[1] 宋明铭 陈凯[1] 王月亮 黄宝亮[1] 丁传波[1] 郑毅男[1] 刘文丛[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《中国免疫学杂志》2016年第11期1614-1620,共7页Chinese Journal of Immunology

基　　金：吉林省科技发展计划项目(No.20150311050YY)

摘　　要：目的:研究二氢槲皮素和二氢杨梅素对H_(22)荷瘤小鼠抑瘤作用。方法:建立小鼠H_(22)移植性肿瘤模型(除空白外)。造模24 h后分成6组:模型组,阳性对照组(环磷酰胺25mg/kg),二氢槲皮素和二氢杨梅素高、低剂量组(30、10mg/kg),除阳性对照组腹腔给药,其余各组均灌胃给药,测定其对H_(22)荷瘤小鼠抑瘤率、免疫器官指数、生化指标和细胞因子含量的影响,并通过HE染色,观察瘤组织内坏死程度。结果:二氢槲皮素和二氢杨梅素高、低剂量组(30mg/kg、10mg/kg)均对肿瘤具有一定抑制作用,且均可提高H_(22)荷瘤小鼠胸腺指数和脾指数,其中二氢槲皮素高剂量组(30mg/kg)抑瘤效果最佳,其抑瘤率为58.8%。二氢槲皮素和二氢杨梅素高、低剂量组均可升高H_(22)荷瘤小鼠细胞因子白细胞介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)的水平,升高超氧化物歧化酶(SOD)的水平,降低丙二醛(MDA)水平。此外,二氢槲皮素和二氢杨梅素高、低剂量组对H_(22)荷瘤小鼠肝肾功能没有毒理作用。结论:二氢槲皮素和二氢杨梅素高、低剂量组均对肿瘤具有一定抑制作用,其中二氢槲皮素组的作用较明显,且呈剂量依赖性,其作用机制可能与增强抗氧化能力、提高机体免疫功能和升高细胞因子水平有关。Objective： To investigate the antitumor effect of Dihydroquercetin and Dihydromyricetin on H_（22）-bearing mice（ in addition to blank）. Methods： To establish the H_（22） implanted hepato-cellular carcinoma animal model which was used to analyze the effect of Dihydroquercetin and Dihydromyricetin on the growth of transplanted tumor. Mice were divided into six groups 24 h after modeling： model,positive control（ gytoxan,25mg/kg）,low-,high-dose（ 10,30mg/kg） Dihydroquercetin and Dihydromyricetin groups. In addition to the positive control group were administered,the other groups were administered orally,the effects of H_（22） on tumor inhibition rate,immune organ index,biochemical index and factor in tumor bearing mice were determined. The degree of necrosis in tumor tissue was observed by Hematoxylin and Eosin（ HE）. Results： Both low（ 10mg/kg） and high（ 30mg/kg） doses of Dihydroquercetin and Dihydromyricetin inhibited tumor growth,could increase the thymus index and spleen index of H_（22）tumor-beating mice. The inhibitory effect was most pronounced（ 58. 8%） with high doses of Dihydroquercetin. Both low and high doses of Dihydroquercetin and Dihydromyricetin also incteased interleukin-2（ IL-2） and tumor necrosis factor-α（ TNF-α） levels,elevated levels of SOD,reduced MDA level. In addition,no toxicological effects were observed on hepatic function and renal function in low and high doses of Dihydroquercetin and Dihydromyricetin. Conclusion： In summary TF and DMY has antitumor effect on H_（22） transplanted tumor in mice,the inhibitory effect was most pronounced with of TF,and was dose dependent. The possible mechanisms may be due to enhanced antioxidant capacity,improve immune function and elevated cytokine levels.

关 键 词：二氢槲皮素 二氢杨梅素 抗肿瘤 

分 类 号：R730.52[医药卫生—肿瘤]