阿魏酸抗LPS诱导的奶牛子宫内膜上皮细胞炎性作用  

Anti-inflammatory effect of ferulic acid on the bovine endometrial epithelial cell treated by LPS

作　　者：张志聪[1] 吕安[1] 宫平[1] 张彤[1] 刘艺林[1] 李德琰 姜晓茜[1] 刘蓓桦 刘凤华[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《北京农学院学报》2016年第4期56-60,共5页Journal of Beijing University of Agriculture

基　　金：国家"十二五"科技支撑计划重点项目(2011BAD34B01)

摘　　要：【目的】为了探明阿魏酸的抗炎机制。【方法】采用组织块培养法获得奶牛子宫内膜上皮细胞。用噻唑蓝(MTT法)检测细胞活力。采用荧光定量PCR方法,分别对对照组、模型组、药物作用高、中、低剂量组在LPS作用3h、6h炎症基因IL-1β、IL-6、IL-8和TNF-α的mRNA变化进行检测。【结果】阿魏酸在一定浓度范围内对细胞增殖无显著影响。药物预处理细胞能够显著降低LPS诱导的IL-1β、IL-6、IL-8和TNF-α的mRNA表达。【结论】阿魏酸能够显著降低LPS刺激细胞后炎症细胞因子的表达,说明阿魏酸是通过抑制炎症因子的表达发挥其抗炎作用。【Objective】The aim of this study was to explore the anti-inflammatory mecchanism of ferulic acid.【Methods】Bovine endometrial epithelial cells（BEEC）were cultured and divided into control group,model group,low,medium,and high LPS groups.The cell viability of BEEC was tested by MTT.RT-PCR was used to detect cytokines（IL-1β,IL-6,IL-8,and TNF-α）mRNA expression of BEEC induced by LPS after 3hand 6h.【Result】There were no significant effects on cell viability among gourps.Ferulic acid significantly reduced IL-1β,IL-6,IL-8,and TNF-αmRNA expression induced by LPS.【Conclusion】Ferulic acid can decrease significantly the cytokines expression of BEEC treated with LPS,showing anti-inflammation effect.

关 键 词：阿魏酸 奶牛子宫内膜上皮细胞 细胞因子 脂多糖 抗炎作用 

分 类 号：S852.4[农业科学—基础兽医学]