荷叶离褶伞发酵液对NaCl胁迫下洋葱根尖细胞有丝分裂的影响  

Effects of Fermentative Fluid of Lyophyllum decastes on Mitosis of Root Tip Cells of Allium cepa L. Under NaCl Stress

作　　者：王兴珍[1] 沈昱彤 魏生龙[3] 卯旭辉[1] 贾秀苹[1] 梁根生[1] 王晓琴[3] 

机构地区：[1]甘肃省农业科学院作物研究所,甘肃兰州730070 [2]白银有色嘉华园林工程有限公司,甘肃白银730400 [3]河西学院农业与生物技术学院,甘肃张掖734000

出　　处：《甘肃农业科技》2016年第11期38-42,共5页Gansu Agricultural Science and Technology

摘　　要：研究了荷叶离褶伞发酵液对NaCl胁迫下洋葱根尖细胞有丝分裂的影响。结果表明:低浓度NaCl(0.05 mol/L)对洋葱根的生长有促进作用,高浓度NaCl(≥0.10 mol/L)对洋葱根的生长有抑制作用,根尖细胞有丝分裂出现异常,且随着NaCl浓度增高和NaCl处理时间延长抑制作用越明显。荷叶离褶伞发酵液对低浓度NaCl(≤0.10 mol/L)短时间处理(24 h)导致的洋葱根尖细胞遗传损伤有一定的修复作用。The root tip of Allium cepa L is treated with different concentrations of NaCl for 24 hours and 48 hours. The results shows that low concentrations of NaCl significantly promoted the growth of Allium cepa L, but higher concentrations（≥0.1 mol/L） played a poisoned role on the growth of Allium cepa L and have a dose-effect relationship. while we cultured the different NaCl treated Allium cepa L using diluted Fermentative Fluid of Lyophyllum decastes for 24 hours, the result shows that diluted Fermentative Fluid of Lyophyllum decastes can repair the genetic damage and promote the growth of a short period of time NaCl treated Allium cepa L （24 h）, such as decreased micronucleus rate and chromosome aberration rate, increased mitotic index, but have a weak role for a long period of time treated NaCl Allium cepa L（48 h）.

关 键 词：荷叶离褶伞发酵液 洋葱 盐胁迫 有丝分裂 修复作用 ALLIUM CEPA L. 

分 类 号：S567.5[农业科学—中草药栽培]