梓醇对内皮细胞炎症因子表达的影响  

Effects of Catalpol on the Inflammatory Factors of Endothelial Cells

作　　者：董倩[1] 孙力[1] 胡雪梅[1] 陈静[1] 周峰[1] 黄琦[1] 徐焱成[1] 

机构地区：[1]武汉大学中南医院内分泌科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2016年第6期884-887,共4页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81370872)

摘　　要：目的:研究梓醇(Catalpol)对高糖高脂诱导的人脐静脉血内皮细胞(HUVECs)炎症反应的影响。方法:体外培养人脐静脉血内皮细胞,用CCK-8法检测不同浓度梓醇溶液对HUVECs细胞增殖活性的影响。将细胞分为空白对照组、高糖高脂组(给予33mmol/L葡萄糖+500μmol/L棕榈酸作用48h)、高糖高脂+1 000mg/L梓醇预处理组(梓醇1 000mg/L预处理24h后,给予33mmol/L葡萄糖+500μmol/L棕榈酸作用48h,以下简称梓醇预处理组),收集细胞上清液,用ELISA检测上清液中的炎症因子TNF-α、IL-6的浓度。结果:梓醇作用于HUVECs细胞的最佳浓度为1 000mg/L。高糖高脂组中TNF-α和IL-6浓度较空白对照组均明显增高(t=7.03、6.68,P均<0.01)。与高糖高脂组比较,梓醇预处理组上清液中炎症因子TNF-α及IL-6浓度均下降(t=-2.87、-3.76,P均<0.05)。结论:高糖高脂可诱导血管内皮细胞炎症反应,而梓醇对高糖高脂诱导的炎症反应有一定的改善作用。Objective：To investigate the effects of catalpol on the inflammatory factors of human umbilical vein endothelial cells（HUVECs）induced by high sugar and high lipid.Methods：HUVECs were cultured in vitro.CCK-8method was used to test cell viability.And then the cells were divided into 3groups：control group,high glucose and high fat group（33mmol/L glucose and 500μmol/L palmitic acid,48h）and1 000mg/L catalpol group.The consistences of tumor necrosis factor-α（TNF-α）and interleukin-6（IL-6）in the supernatant were detected by enzyme-linked immunosorbent assay（ELISA）to observe the inflammation reaction.Results：The consistences of TNF-αand IL-6in high sugar and high lipid group were obviously higher than those in control group（t=7.03,t=6.68,P〈0.01）.The consistences of TNF-α（t=-2.87,P〈0.05）and IL-6（t=-3.76,P〈0.05）in 1 000mg/L catalpol group were lower than those in high sugar and high lipid group.Conclusion：High sugar and high lipid can induce inflammatory reaction.Catalpol,in some degree,inhibits the inflammation of HUVECs induced by high sugar and high lipid.

关 键 词：梓醇 血管内皮细胞 肿瘤坏死因子-Α 白细胞介素-6 

分 类 号：R364.5[医药卫生—病理学] R587.1[医药卫生—基础医学]