不同ELISA试剂盒检测牛结核病效果比较  

COMPARISON OF DIFFERENT ELISA KITS FOR DETECTION OF BOVINE γ-IFN

作　　者：邵红霞[1,2] 赵巍[1,2] 黄健[1,2] 钱琨[1,2] 叶建强[1,2] 秦爱建[1,2] 

机构地区：[1]扬州大学兽医学院禽类预防医学教育部重点实验室江苏省动物预防医学重点实验室,扬州225009 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心,扬州225009

出　　处：《中国动物传染病学报》2016年第5期29-32,共4页Chinese Journal of Animal Infectious Diseases

基　　金：江苏省科技支撑计划(BE2013391);江苏省优势学科项目

摘　　要：本研究对自行研制的检测牛γ干扰素的双夹心ELISA试剂盒与商品化试剂盒进行了比较。用自行研制的双夹心ELISA试剂盒对1255份经牛PPD刺激的牛全血培养上清样品进行了检测,结果发现94份阳性样品,检测阳性率为7.5%。同时用进口的ELISA试剂盒对其中的472份样品进行了对比检测,两者阳性符合率为97.6%,阴性符合率为96.4%,总符合率为96.6%。比较检测结果证明,自行研制的检测牛γ干扰素的双夹心ELISA试剂盒有很好的应用前景。To select high quality diagnostic kit for bovine tuberculosis, the domestic sandwich ELISA developed in our laboratory and a commercial ELISA kit were compared for detection of bovine γ-IFN in whole blood stimulated with BPPD. The positive percentage was 7.5%（94/1255） with the domestic sandwich ELISA. Furthermore, 472 of these 1255 samples were tested in both ELISA kits. Our data showed that the positive, negative and total agreement between the two ELISAs were 97.6%, 96.4% and 96.6%, respectively. The direct comparison demonstrated that the domestic sandwich ELISA developed in our laboratory had promising application for detection of bovine γ-IFN.

关 键 词：检测牛γ干扰素 双夹心ELISA 比较 

分 类 号：S852.618[农业科学—基础兽医学]