转化生长因子β1和基质金属蛋白酶2在糖尿病性白内障大鼠晶状体上皮细胞中的表达及意义  

Expression and its significance of transforming growth factor β1 and matrix metalloproteinases 2 in lens epithelial cells of diabetic cataract rats

作　　者：温积权[1] 洪明胜[1] 张杨[2] 

机构地区：[1]浙江医院眼科,浙江杭州310013 [2]温州医科大学附属第一医院眼科,浙江温州325000

出　　处：《中华全科医学》2016年第12期2042-2044,2094,共4页Chinese Journal of General Practice

基　　金：浙江省温州市2015年公益性科技计划项目(Y2015-0271)

摘　　要：目的探讨糖尿病性白内障大鼠晶状体上皮细胞(lens epithelial cells,LECs)中转化生长因子β1(transforming growth factorβ1,TGF-β1)和基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)的表达情况及其意义。方法将28只SD大鼠随机分为白内障组和对照组,建立糖尿病性白内障大鼠模型,观察2组大鼠LECs变化,测定2组大鼠LECs中TGF-β1和MMP-2表达情况。结果 HE染色:白内障组大鼠LECs形态异常,排列紊乱,核大小不均匀,胞质疏松;对照组大鼠LECs正常。白内障组大鼠2周末和4周末LECs中TGF-β1蛋白和MMP-2蛋白平均光密度(0.045±0.002、0.075±0.006;0.028±0.003、0.055±0.006)均高于对照组(0.006±0.001、0.006±0.002;0.000、0.000,均P<0.05),白内障组大鼠4周末LECs中TGF-β1蛋白和MMP-2蛋白平均光密度高于2周末(P<0.05)。白内障大鼠LECs中TGF-β1蛋白和MMP-2蛋白呈正相关(r=0.915,P=0.004)。结论糖尿病性白内障的发生发展可能和LECs中TGF-β1蛋白、MMP-2蛋白水平升高有关,糖尿病性白内障LECs中TGF-β1蛋白和MMP-2蛋白呈正相关。Objective To investigate the expression and its significance of transforming growth factor β1 （ TGF-β1 ） and matrix metalloproteinases 2 （MMP-2） in lens epithelial cells （ LECs ） of diabetic cataract rats. Methods The 28 SD rats were randomly divided into cataract group and control group, and the rat model of diabetic cataract was established. The rats LECs change in two groups were detected. The expression of TGF-β1 and MMP- 2 in LECs of the two groups were detected. Results HE staining ： the LECs of cataract group rats morphological was abnormal , derangement was disordered, nuclear size was uneven, and cytoplasm was loose;the LECs of control group rats was normal. The mean optical density of TGF-β1 protein and MMP-2 protein in LECs at 2 weekend and 4 weekend in cataract group rats （ 0. 0447± 0. 002, 0. 075 ± 0.006 ;0. 028 ± 0. 003,0. 055 ± 0. 006） were higher than that of the control group （0. 006±0. 001,0. 0063 ±0.002 ;P =0. 000,0. 000）,the mean optical density of TGF-β1 protein and MMP-2 protein in LECs at 4 weekend were higher than that of 2 weekend in cataract group rats（P 〈0.05 ）. TGF-β1 protein and MMP-2 protein in LECs of cataract group rats was positively correlated（ r = 0.915 ,P = 0. 004）. Conclusion Development of diabetic cataract may be correlated to the exaltation of TGF-β1 protein and MMP-2 protein levels of LECs, and the TGF-β1 protein and MMP-2 protein in LECs of diabetic cataract were positively correlated.

关 键 词：糖尿病性白内障 大鼠 晶状体上皮细胞 基质金属蛋白酶2 转化生长因子Β1 

分 类 号：R776.1[医药卫生—眼科] R587.2[医药卫生—临床医学]